Marshall Laboratory of Biomedical Engineering, Shenzhen Key Laboratory for Nano-Biosensing Technology, School of Biomedical Engineering, Shenzhen University Medical School, Shenzhen University, Guangdong, 518060, China.
Marshall Laboratory of Biomedical Engineering, Shenzhen Key Laboratory for Nano-Biosensing Technology, School of Biomedical Engineering, Shenzhen University Medical School, Shenzhen University, Guangdong, 518060, China.
Anal Chim Acta. 2023 May 15;1255:341101. doi: 10.1016/j.aca.2023.341101. Epub 2023 Mar 21.
The interstitial fluid (ISF) contains rich bioinformation for disease diagnosis and healthcare monitoring. However, the efficient sampling and detection of the biomolecules in ISF is still challenging. Herein, we develop a facile but versatile ISF analysis platform by combining controllable hollow microneedles (HMNs) and elaborate microfluidic paper-based analytical devices (μPADs). The HMNs and μPADs was fixed in a bottom PDMS layer. A top PDMS layer containing a cylindrical cavity to produce negative pressure for sampling was packaged on the bottom PDMS layer. The HMNs enable efficient and swift sampling of sufficient ISF to the μPADs through one-touch finger operation without extra manipulations. The μPADs realized to simultaneously detect glucose and lactic acid in the detection area to produce chromogenic agents and analyzed by the self-programed RGB application (APP) in smartphones. The HMN microfluidic paper-based chip provides a point-of-care platform for accurate detection of biomolecules in ISF, holding great promise in the development of wearable device.
间质液（ISF）中含有丰富的生物信息，可用于疾病诊断和健康监测。然而，高效地采集和检测 ISF 中的生物分子仍然具有挑战性。在此，我们通过结合可控的中空微针（HMN）和精心设计的微流控纸基分析器件（μPAD），开发了一种简单但通用的 ISF 分析平台。HMN 和 μPAD 固定在底部 PDMS 层上。顶部 PDMS 层包含一个圆柱形腔室，用于产生负压进行采样，并封装在底部 PDMS 层上。通过一键式手指操作，HMN 可高效、快速地采集足够的 ISF 到 μPAD 上，无需额外操作。μPAD 可以在检测区域同时检测葡萄糖和乳酸，产生显色剂，并通过智能手机中的自编程 RGB 应用程序（APP）进行分析。HMN 微流控纸基芯片为准确检测 ISF 中的生物分子提供了一个即时检测平台，在可穿戴设备的发展中具有广阔的前景。
