Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Chemistry, Ferdowsi University of Mashhad, Mashhad, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Feb 15;267(Pt 2):120488. doi: 10.1016/j.saa.2021.120488. Epub 2021 Oct 13.
In this research, a facile fluorescence aptasensor was designed for the ultrasensitive determination of ethanolamine (ETA) as an aliphatic amino alcohol molecule with harmful side effects for human health. Zeolitic imidazolate framework-8 (ZIF-8) as an efficient metal-organic framework was applied to quench the fluorescence emission of the FAM-labeled ETA aptamer. The presence of ETA could recover the fluorescence response under the optimal experimental conditions. ETA could be sensitively detected by the designed aptasensor in the linear concentration range of 0.1 nM-20 µM with the detection limit of 14.38 pM. The designed aptasensor was utilized to determine ETA in milk and serum samples with the detection limits of 17.86 and 15.21 pM, respectively. The aptasensor with the remarkable features of high sensitivity, simplicity and feasibility holds the supreme potential to construct a low-cost portable sensing method for food safety control, human diagnostics, and environmental monitoring with coupling to the microfluidic paper-based devices.
在这项研究中，设计了一种简便的荧光适体传感器，用于超灵敏测定乙醇胺（ETA），作为一种具有潜在健康危害的脂肪族氨基醇分子。沸石咪唑酯骨架-8（ZIF-8）作为一种高效的金属有机骨架，用于猝灭 FAM 标记的 ETA 适体的荧光发射。在最佳实验条件下，ETA 的存在可以恢复荧光响应。在 0.1 nM-20 µM 的线性浓度范围内，通过设计的适体传感器可以灵敏地检测 ETA，检测限为 14.38 pM。该设计的适体传感器可用于测定牛奶和血清样品中的 ETA，检测限分别为 17.86 和 15.21 pM。该适体传感器具有灵敏度高、简单、可行等显著特点，有望与微流控纸基器件相结合，构建用于食品安全控制、人类诊断和环境监测的低成本便携式传感方法。
