Department of New Pediatrics, Xiangya Hospital of Central South University, Changsha 410008, PR China.
Chongqing Key Laboratory for Pharmaceutical Metabolism Research, The Key Laboratory of Biochemistry and Molecular Pharmacology, College of Pharmacy, College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 400016, PR China; Department of New Pediatrics, Xiangya Hospital of Central South University, Changsha 410008, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Feb 15;267(Pt 2):120524. doi: 10.1016/j.saa.2021.120524. Epub 2021 Oct 23.
Hydrogen polysulfide (HS, n > 1) is an important active sulfur molecule (RSS) in organisms, which have been considered to be involved in redox signaling and cytoprotective processes. In this work, in order to quickly and accurately detect HS in biosystems, 2-fluoro-5-nitrobenzoic ester was used as the response moiety for HS, and the FRET strategy was adopted to effectively connect the donor (6-hydroxy-2-naphthoic acid) and acceptor (4-substituted-1,8-naphthalimide) to construct a new ratiometric HS fluorescent probe NPNA-HS. NPNA-HS exhibited a more than ∼ 8.0-fold ratio enhancement towards HS at I/I and a very high sensitivity with a very low detection limit of 40.3 nM. Impressive, NPNA-HS was further used for fluorescence imaging of HS in living cells and zebrafish, which showed high-clear ratiometric images. Therefore, we have demonstrated that NPNA-HS could be applied for ratiometric images of endogenous HS in living biosystems and provide a powerful molecular tool for evaluating the physiological and pathological functions of HS.
氢多硫化物（HS，n > 1）是生物体中一种重要的活性硫分子（RSS），被认为参与氧化还原信号和细胞保护过程。在这项工作中，为了快速、准确地检测生物体系中的 HS，我们将 2-氟-5-硝基苯甲酸酯用作 HS 的响应部分，并采用 FRET 策略将供体（6-羟基-2-萘甲酸）和受体（4-取代-1,8-萘二甲酰亚胺）有效地连接起来，构建了一种新的比率型 HS 荧光探针 NPNA-HS。NPNA-HS 对 HS 的比率增强超过 ∼8.0 倍，具有非常高的灵敏度，检测限低至 40.3 nM。令人印象深刻的是，NPNA-HS 进一步用于活细胞和斑马鱼中 HS 的荧光成像，显示出高清晰的比率图像。因此，我们已经证明 NPNA-HS 可用于活生物体系中内源性 HS 的比率成像，并为评估 HS 的生理和病理功能提供了一种强大的分子工具。
