Department of Radiology, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, 571199, China.
Department of Radiotherapy, The First Affiliated Hospital of Hainan Medical University, Key Laboratory of Emergency and Trauma, Ministry of Education, Key Laboratory of Hainan Trauma and Disaster Rescue, College of Emergency and Trauma, Hainan Medical University, Haikou, 571199, China.
Anal Chim Acta. 2022 Sep 15;1226:340288. doi: 10.1016/j.aca.2022.340288. Epub 2022 Aug 22.
As a member of reactive sulfur molecules, hydrogen polysulfide (HS) plays a vital role in cell protection, anti-oxidative stress and regulation of redox signaling. The highly selective and sensitive detection of HS was still challenging due to its special nucleophilic and electrophilic reactivity. By incorporating phenyl 2-(benzoylthio) benzoate into semi-naphthofluorescein, we developed a novel red emissive fluorescent probe SNAFL-HS for the detection of a representative HS (e. g. HS). The addition of HS would rapidly trigger SNAFL-HS to produce significant turn-on fluorescence signal changes at 626 nm with a linear response over a range of 2-30 μM and a detection limit of 16 nM. SNAFL-HS was capable of mapping exogenous and endogenous HS in living cells and zebrafish. Moreover, SNAFL-HS was applied to detect endogenous HS under atorvastatin stimulation. The present study demonstrated that SNAFL-HS potentially served as a promising tool for interrogating HS functions in biological systems.
作为活性硫分子的一员，氢多硫化物（HS）在细胞保护、抗氧化应激和氧化还原信号调节中发挥着重要作用。由于其特殊的亲核和亲电反应性，HS 的高选择性和灵敏检测仍然具有挑战性。我们将苯 2-（苯甲酰硫代）苯甲酸酯纳入半萘荧光素中，开发了一种新型的红色发射荧光探针 SNAFL-HS，用于检测代表性的 HS（例如 HS）。HS 的加入会迅速触发 SNAFL-HS 在 626nm 处产生显著的开环荧光信号变化，在 2-30μM 的范围内呈线性响应，检测限为 16nM。SNAFL-HS 能够在活细胞和斑马鱼中定位外源性和内源性 HS。此外，SNAFL-HS 还可用于检测阿托伐他汀刺激下的内源性 HS。本研究表明，SNAFL-HS 可能成为研究生物体系中 HS 功能的一种有前途的工具。
