Ma Yingying, Xu Zhencai, Sun Qi, Wang Linlin, Liu Heng, Yu Fabiao
Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China; Key Laboratory of Emergency and Trauma, Ministry of Education, Key Laboratory of Hainan Trauma and Disaster Rescue, The First Affiliated Hospital of Hainan Medical University, Institute of Functional Materials and Molecular Imaging, College of Emergency and Trauma, Hainan Medical University, Haikou 571199, China.
Key Laboratory of Emergency and Trauma, Ministry of Education, Key Laboratory of Hainan Trauma and Disaster Rescue, The First Affiliated Hospital of Hainan Medical University, Institute of Functional Materials and Molecular Imaging, College of Emergency and Trauma, Hainan Medical University, Haikou 571199, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Feb 15;247:119105. doi: 10.1016/j.saa.2020.119105. Epub 2020 Oct 24.
Hydrogen polysulfides (HS n ≥ 2) is recently regarded as a potential signaling molecule which shows a higher efficiency than hydrogen sulfides (HS) in regulating enzymes and ion channels. However, the development of specific fluorescent probes for HS with long-wavelength emission (>600 nm) are still rare. In this work, a semi-naphthorhodafluor-based red-emitting fluorescent probe SNARF-HS containing a phenyl 2-(benzoylthio) benzoate responsive unit was constructed. SNARF-HS was capable of selectively detecting HS over other reactive sulfur species. Treatment with HS would result in a > 1000-fold fluorescence enhancement within 10 min. SNARF-HS showed a low limit of detection down to 6.7 nM, and further enabled to visualize exogenous/endogenous HS in living A549 cells and zebrafish.
多硫化氢（HSn，n≥2）最近被认为是一种潜在的信号分子，在调节酶和离子通道方面比硫化氢（HS）具有更高的效率。然而，用于发射长波长（>600 nm）的HS的特异性荧光探针仍然很少。在这项工作中，构建了一种基于半萘罗丹明的红色发射荧光探针SNARF-HS，其含有苯基2-(苯甲酰硫基)苯甲酸酯响应单元。SNARF-HS能够选择性地检测HS，而不是其他活性硫物种。用HS处理会在10分钟内导致荧光增强超过1000倍。SNARF-HS的检测下限低至6.7 nM，并进一步能够可视化活的A549细胞和斑马鱼中的外源性/内源性HS。
