College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.
Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China.
Anal Chem. 2022 Jun 21;94(24):8792-8801. doi: 10.1021/acs.analchem.2c01650. Epub 2022 Jun 6.
Hydrogen polysulfide (HS), derived from hydrogen sulfide (HS), has attracted increasing attention, which is suggested to be the actual signal molecule instead of HS in physiological and pathological processes. Reversible detection of HS through a NIR-excitable fluorescence probe is an effective means to understand its functions but is quite challenging. Herein, we reported a NIR-excitable ratiometric nanoprobe for the reversible detection of HS based on luminescence resonance energy transfer principle with upconversion nanoparticles as the energy donor and an organic molecule, SiR1, as the energy acceptor and reversible recognition unit of HS. The as-prepared nanoprobe exhibited high selectivity and fast response for the reversible detection of HS, which can monitor the formation and consumption of endogenous HS in living cells. Because of the reduced autofluorescence by NIR excitation, it was successfully applied for tracking the fluctuation of HS concentration of mice in physiological and pathological processes including inflammation and liver injury.
氢多硫化物（HS）来源于硫化氢（HS），越来越受到关注，它被认为是生理和病理过程中 HS 的实际信号分子。通过近红外激发荧光探针可逆检测 HS 是了解其功能的有效手段，但极具挑战性。本文基于上转换纳米粒子作为能量供体和有机分子 SiR1 作为能量受体和 HS 的可逆识别单元，报道了一种用于 HS 可逆检测的近红外激发比率型纳米探针。该制备的纳米探针对 HS 的可逆检测具有高选择性和快速响应，可以监测活细胞内内源性 HS 的形成和消耗。由于近红外激发减少了自发荧光，它成功地应用于跟踪包括炎症和肝损伤在内的生理和病理过程中 HS 浓度的波动。
