College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530005, PR China.
College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530005, PR China.
Vet Parasitol. 2021 Dec;300:109585. doi: 10.1016/j.vetpar.2021.109585. Epub 2021 Sep 23.
Fasciolosis is harmful to ruminant husbandry worldwide. Given the superficial survey on Fasciolosis infection in Guangxi, the main buffalo breeding area in China, an in-depth investigation in the infection of buffaloes in Nanning, the capital of Guangxi Zhuang Autonomous Region, with Fasciola (Platyhelminthes: Trematoda: Digenea) species will provide a theoretical support for the control and prevention of Fasciolosis infection in buffaloes. Five water buffalo livers were collected from an abattoir in Nanning every 2 weeks from June 2018 to April 2019, and a total of 101 livers were obtained. All livers were then dissected to observe the liver lesions caused by the flukes. Afterwards, Fasciola spp. collected from Fasciolosis-infected livers were numbered and measured. Then, the livers infected with more than 3 flukes were marked, and 3 flukes were picked from each liver according to their morphological differences, such as body length (BL), body maximum width (BW) and length-width ratio (BL/BW). Moreover, these Fasciola spp. worms were selected for molecular biological analysis. The second internal transcribed spacer (ITS-2) of nuclear ribosomal DNA (rDNA) was amplified by polymerase chain reaction (PCR) and sequenced. Finally, sequential and phylogenetic analyses were also performed. The infection rate was 38.6 % according to anatomic examination, and the livers infected by Fasciola spp. were damaged seriously. The principal manifestations were the enlargement of the liver and protrusion of the bile ducts. In some cases, the bile duct wall became inflamed and rough, in which some sediment such as phosphate could be easily found. After dissection, 1243 Fasciola spp. flukes were collected from 39 out of 101 livers. The morphometric measurements obtained from the present study showed that the BL/BW ranged from 1.42-10.25. However, it might vary considerably among different geographical locations and could not be used as an accurate method for the identification of Fasciola spp.. Analysis of the ITS-2 sequences showed that 83 out of 87 flukes had 100 % homology with each other, and the other 4 flukes with 99.3 % homology possessed a nucleotide polymorphism. A unique position (271) was detected in flukes in Nanning isolates. Phylogenetic analysis indicated that all the flukes were Fasciola gigantica, and no Fasciola hepatica or the intermediate form was found in this study.
片形吸虫病对世界范围内的反刍动物养殖业造成危害。鉴于对中国主要水牛养殖区广西片形吸虫病感染的初步调查，对广西壮族自治区首府南宁市水牛的感染进行深入调查，用 Fasciola（扁形动物门：吸虫纲：复殖目）物种提供了控制和预防水牛感染片形吸虫病的理论依据。从 2018 年 6 月到 2019 年 4 月，每隔两周从南宁市的一个屠宰场收集 5 头水牛的肝脏，共获得 101 个肝脏。然后对所有肝脏进行解剖，观察肝吸虫引起的肝损伤。然后，对来自感染片形吸虫病肝脏的 Fasciola spp.进行编号和测量。然后，对感染 3 条以上吸虫的肝脏进行标记，根据形态差异（如体长（BL）、体最大宽度（BW）和体长宽度比（BL/BW））从每个肝脏中取出 3 条 Fasciola spp.吸虫。此外，这些 Fasciola spp. 蠕虫被选择进行分子生物学分析。通过聚合酶链反应（PCR）扩增核核糖体 DNA（rDNA）的第二内部转录间隔区（ITS-2）并进行测序。最后，还进行了序列和系统发育分析。解剖检查的感染率为 38.6%，感染 Fasciola spp.的肝脏严重受损。主要表现为肝脏肿大和胆管突出。在某些情况下，胆管壁发炎和粗糙，其中很容易发现一些沉淀物，如磷酸盐。解剖后，从 101 个肝脏中的 39 个肝脏中收集了 1243 条 Fasciola spp.吸虫。本研究的形态测量结果表明，BL/BW 范围为 1.42-10.25。然而，它在不同的地理位置之间可能有很大的差异，不能作为 Fasciola spp.鉴定的准确方法。ITS-2 序列分析表明，87 条吸虫中的 83 条彼此具有 100%的同源性，另外 4 条具有 99.3%的同源性的吸虫具有核苷酸多态性。在南宁分离株的吸虫中检测到一个独特的位置（271）。系统发育分析表明，所有的吸虫都是巨片形吸虫，本研究未发现肝片形吸虫或中间型。
