Centre of Advanced Materials (CAM), Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia; Department of Chemical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Centre of Advanced Materials (CAM), Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia; Department of Chemical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Int J Biol Macromol. 2021 Dec 15;193(Pt B):1522-1531. doi: 10.1016/j.ijbiomac.2021.10.215. Epub 2021 Nov 2.
The investigation of protein-nanoparticle interactions contributes to the understanding of nanoparticle bio-reactivity and creates a database of nanoparticles for use in nanomedicine, nanodiagnosis, and nanotherapy. In this study, hen's egg white was used as the protein source to study the interaction of proteins with sulphuric acid hydrolysed nanocellulose (CNC). Several techniques such as FTIR, zeta potential measurement, UV-vis spectroscopy, compressive strength, TGA, contact angle and FESEM provide valuable information in the protein-CNC interaction study. The presence of a broader peak in the 1600-1050 cm range of CNC/egg white protein FTIR spectrum compared to the 1600-1050 cm range of CNC sample indicated the binding of egg white protein to CNC surface. The contact angle with the glass surface decreased with the addition of CNC to egg white protein. The FESEM EDX spectra showed a higher amount of N and Na on the surface of CNC. It indicates the density of protein molecules higher around CNC. The zeta potential of CNC changed from -26.7 ± 0.46 to -21.7 ± 0.2 with the introduction of egg white protein due to the hydrogen bonding, polar bonds and electrostatic interaction between surface CNC and protein. The compressive strength of the egg white protein films increased from 0.064 ± 0.01 to 0.36 ± 0.02 MPa with increasing the CNC concentration from 0 to 4.73% (w/v). The thermal decomposition temperature of CNC/egg white protein decreased compared to egg white protein thermal decomposition temperature. According to UV-Vis spectroscopy, the far-UV light (207-222nm) absorption peak slightly changed in the CNC/egg white protein spectrum compared to the egg white protein spectrum. Based on the results, the observations of protein nanoparticle interactions provide an additional understanding, besides the theoretical simulations from previous studies. Also, the results indicate to aim CNC for the application of nanomedicine and nanotherapy. A new insight given by us in this research assumes a reasonable solution to these crucial applications.
蛋白质-纳米颗粒相互作用的研究有助于理解纳米颗粒的生物反应性，并为纳米医学、纳米诊断和纳米治疗创建纳米颗粒数据库。在这项研究中，使用鸡蛋蛋白作为蛋白质来源来研究蛋白质与硫酸水解纳米纤维素（CNC）的相互作用。傅里叶变换红外光谱（FTIR）、zeta 电位测量、紫外可见光谱、压缩强度、热重分析（TGA）、接触角和场发射扫描电子显微镜（FESEM）等多种技术提供了蛋白质-CNC 相互作用研究中的有价值的信息。与 CNC 样品的 1600-1050cm 范围内的 FTIR 光谱相比，CNC/蛋清蛋白 FTIR 光谱中 1600-1050cm 范围内出现了更宽的峰，表明蛋清蛋白结合到 CNC 表面。随着 CNC 加入到蛋清蛋白中，与玻璃表面的接触角减小。FESEM EDX 光谱显示 CNC 表面的 N 和 Na 含量更高。这表明 CNC 周围的蛋白质分子密度更高。由于表面 CNC 和蛋白质之间的氢键、极性键和静电相互作用，CNC 的 zeta 电位从-26.7±0.46 变为-21.7±0.2，随着蛋清蛋白的引入而发生变化。随着 CNC 浓度从 0 增加到 4.73%（w/v），蛋清蛋白膜的压缩强度从 0.064±0.01 增加到 0.36±0.02MPa。与蛋清蛋白热分解温度相比，CNC/蛋清蛋白的热分解温度降低。根据紫外可见光谱，与蛋清蛋白光谱相比，CNC/蛋清蛋白光谱中远紫外光（207-222nm）吸收峰略有变化。根据这些结果，除了以前研究中的理论模拟外，蛋白质-纳米颗粒相互作用的观察提供了额外的理解。此外，结果表明 CNC 可用于纳米医学和纳米治疗。我们在这项研究中提供的新见解为这些关键应用提供了合理的解决方案。
