Jerez-Olate Christian, Araya Nancy, Alcántara Raúl, Luengo Luis, Bello-Toledo Helia, González-Rocha Gerardo, Sánchez-Sanhueza Gabriela
Department of Restorative Dentistry, Discipline of Endodontics, Faculty of Dentistry, Universidad de Concepción, Concepción, Chile.
Department of Public Health and Preventive Dentistry, School of Dentistry, Universidad de Concepción, Concepción, Chile.
Aust Endod J. 2022 Dec;48(3):465-472. doi: 10.1111/aej.12587. Epub 2021 Nov 6.
The aim of this in vitro study was to evaluate the antibacterial activity of calcium silicate repair cements and sealers against a dual-species planktonic aerobic model with different aging times and the ability to inhibit the formation of a mature 21-day-old multispecies anaerobic biofilm. The antibacterial activity of ProRoot MTA, MTA Angelus, Biodentine, BioRoot RCS and TotalFill BC sealer against a dual-species aerobic planktonic model, as well as measuring how materials were affected by aging, was evaluated using the Modified Direct Contact Test. Subsequently, the ability to inhibit the formation of a mature multispecies anaerobic biofilm was evaluated using scanning electron microscopy complemented with confocal laser scanning microscopy. Biodentine and BioRoot RCS had higher antibacterial action, and Biodentine was able to maintain its antibacterial action after a prolonged aging period in vitro. Calcium silicate repair cement MTA ProRoot and Biodentine had higher antibiofilm action.
本体外研究的目的是评估硅酸钙修复水泥和封闭剂对具有不同老化时间的双菌种浮游需氧模型的抗菌活性，以及抑制成熟的21日龄多菌种厌氧生物膜形成的能力。使用改良直接接触试验评估了ProRoot MTA、MTA Angelus、Biodentine、BioRoot RCS和TotalFill BC封闭剂对双菌种需氧浮游模型的抗菌活性，以及测量材料如何受到老化的影响。随后，使用扫描电子显微镜结合共聚焦激光扫描显微镜评估了抑制成熟多菌种厌氧生物膜形成的能力。Biodentine和BioRoot RCS具有更高的抗菌作用，并且Biodentine在体外长时间老化后仍能保持其抗菌作用。硅酸钙修复水泥MTA ProRoot和Biodentine具有更高的抗生物膜作用。
