Ruiz-Linares Matilde, Fedoseev Vsevolod, Solana Carmen, Muñoz-Sandoval Cecilia, Ferrer-Luque Carmen María
Department of Stomatology, University of Granada, 18071 Granada, Spain.
Instituto de Investigación Biosanitaria, 18012 Granada, Spain.
Materials (Basel). 2024 Aug 8;17(16):3937. doi: 10.3390/ma17163937.
Using endodontic sealers with long-term antimicrobial properties can increase the success of endodontic treatment. This study aimed to assess the antimicrobial activity over time of two calcium silicate (CS)-based sealers, AH Plus Bioceramic and BioRoot RCS, and to compare them with an epoxy resin-based sealer, AH Plus Jet, against mature polymicrobial biofilms grown on human radicular dentin.
The antimicrobial activity of the sealers was tested using a direct contact test after 1 and 6 weeks of contact with the biofilms. Cell viability was determined by the adenosine triphosphate (ATP) method and flow cytometry (FC). The results of the ATP test were analyzed using an ANOVA with Welch's correction, followed by the Games-Howell test. The number of cells with damaged membranes obtained by FC in each period was compared by means of an ANOVA and Duncan's test. For the comparison between times, a Student's -test was used.
Globally, after a week of contact, the epoxy resin-based sealer obtained the best results. However, at 6 weeks, the two CSs showed the highest antimicrobial efficacy, with a significant increase in this activity over time.
Calcium silicate-based sealers exert long-term antimicrobial activity against endodontic biofilms.
使用具有长期抗菌性能的根管封闭剂可提高根管治疗的成功率。本研究旨在评估两种硅酸钙（CS）基封闭剂AH Plus Bioceramic和BioRoot RCS随时间的抗菌活性，并将它们与一种环氧树脂基封闭剂AH Plus Jet进行比较，以对抗在人牙根牙本质上生长的成熟多微生物生物膜。
在与生物膜接触1周和6周后，使用直接接触试验测试封闭剂的抗菌活性。通过三磷酸腺苷（ATP）法和流式细胞术（FC）测定细胞活力。ATP试验结果采用带有Welch校正的方差分析进行分析，随后进行Games-Howell检验。通过方差分析和Duncan检验比较各时期FC获得的细胞膜受损细胞数量。对于不同时间点之间的比较，使用Student's检验。
总体而言，接触1周后，环氧树脂基封闭剂取得了最佳效果。然而，在6周时，两种CS基封闭剂显示出最高的抗菌效果，且该活性随时间显著增加。
硅酸钙基封闭剂对根管生物膜具有长期抗菌活性。
