Departamento de Odontología Restauradora, Facultad de Odontología, Universidad de Concepción, Concepción, Chile.
Departamento de Salud Pública, Facultad de Odontología, Universidad de Concepción, Concepción, Chile.
Clin Exp Dent Res. 2023 Apr;9(2):322-331. doi: 10.1002/cre2.714. Epub 2023 Mar 2.
An ideal filling material should hermetically seal the communication pathways between the canal system and surrounding tissues. Therefore, during the last few years, the development of obturation materials and techniques to create optimal conditions for the proper healing of apical tissues has been a focus of interest. The effects of calcium silicate-based cements (CSCs) on periodontal ligament cells have been investigated, and promising results have been obtained. To date, there are no reports in the literature that have evaluated the biocompatibility of CSCs using a real-time live cell system. Therefore, this study aimed to evaluate the real-time biocompatibility of CSCs with human periodontal ligament cells (hPDLCs).
hPDLC were cultured with testing media of endodontic cements for 5 days: TotalFill-BC Sealer, BioRoot RCS, Tubli-Seal, AH Plus, MTA ProRoot, Biodentine, and TotalFill-BC RRM Fast Set Putty. Cell proliferation, viability, and morphology were quantified using real-time live cell microscopy with the IncuCyte S3 system. Data were analyzed using the one-way repeated measures (RM) analysis of variance multiple comparison test (p < .05).
Compared to the control group, cell proliferation in the presence of all cements was significantly affected at 24 h (p < .05). ProRoot MTA and Biodentine lead to an increase in cell proliferation; there were no significant differences with the control group at 120 h. In contrast, Tubli-Seal and TotalFill-BC Sealer inhibited cell growth in real-time and significantly increased cell death compared to all groups. hPDLC co-cultured with sealer and repair cements showed a spindle-shaped morphology except with cements Tubli-Seal and TotalFill-BC Sealer where smaller and rounder cells were obtained.
The biocompatibility of the endodontic repair cements performed better than the sealer cements, highlighting the cell proliferation of the ProRoot MTA and Biodentine in real-time. However, the calcium silicate-based TotalFill-BC Sealer presented a high percentage of cell death throughout the experiment similar to that obtained.
理想的填充材料应能密封根管系统与周围组织之间的连通途径。因此，在过去的几年中，开发能够为根尖组织的适当愈合创造最佳条件的封闭材料和技术一直是研究的重点。已经研究了基于硅酸钙的水泥（CSC）对牙周韧带细胞的影响，并取得了有希望的结果。迄今为止，尚无文献报道使用实时活细胞系统评估 CSC 的生物相容性。因此，本研究旨在评估 CSC 与人类牙周韧带细胞（hPDLC）的实时生物相容性。
将 hPDLC 与牙髓水泥的测试培养基一起培养 5 天：TotalFill-BC 密封剂、BioRoot RCS、Tubli-Seal、AH Plus、MTA ProRoot、Biodentine 和 TotalFill-BC RRM Fast Set 腻子。使用 IncuCyte S3 系统的实时活细胞显微镜定量细胞增殖、活力和形态。使用单向重复测量（RM）方差分析多比较检验（p<0.05）分析数据。
与对照组相比，所有水泥存在时的细胞增殖在 24 小时时受到显著影响（p<0.05）。ProRoot MTA 和 Biodentine 导致细胞增殖增加；与对照组相比，120 小时时没有显著差异。相比之下，Tubli-Seal 和 TotalFill-BC 密封剂实时抑制细胞生长，并与所有组相比显著增加细胞死亡。与密封剂和修复水泥共培养的 hPDLC 呈梭形形态，除了 Tubli-Seal 和 TotalFill-BC 密封剂外，获得的细胞较小且呈圆形。
牙髓修复水泥的生物相容性优于密封剂，突出了 ProRoot MTA 和 Biodentine 的实时细胞增殖。然而，基于硅酸钙的 TotalFill-BC 密封剂在整个实验过程中表现出高细胞死亡率，与获得的死亡率相似。
