State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Int J Biol Macromol. 2021 Dec 15;193(Pt B):1659-1668. doi: 10.1016/j.ijbiomac.2021.11.003. Epub 2021 Nov 4.
The GOBP2 protein has a unique function in the yellow peach moth (Conogethes punctiferalis (Guenée)). Several general odorant-binding proteins (GOBPs) have been identified in various lepidopteran species, but the functional difference between GOBP1 and GOBP2 in recognition of host plant odorants is still unknown. The functions of GOBP1 and GOBP2 in the yellow peach moth were evaluated in this study by using the CRISPR-Cas9 system. The results revealed the importance of GOBP2 in the olfaction mechanism in the yellow peach moth. The perception of the GOBP1-knockout larvae toward feeding decreased but did not reach a significant level while knocking out the GOBP2 and GOBP1/2 genes resulted in huge differences. On the other hand, electroantennograms (EAGs) and wind tunnel tests showed that the sensitivity of GOBP2 knockout adults to odorants decreased more than that of GOBP1 knockout individuals. The results of STRING database text mining grabbed our attention in protein-protein interaction studies. In this research, we first proved the existence of physical interactions between GOBPs and chemosensory proteins (CSPs) through the surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) methods. Interestingly, GOBP1 and GOBP2 could not interact with each other, but they could interact with CSPs. The interaction results indicated that GOBP2 could physically interact with CSP15, CSP5, and OBP17, whereas GOBP1 could bind only with CSP5 and CSP10, and its association constant (k) was also more substantial than that of GOBP1. These results strongly suggest the importance of the function of GOBP2 in the perception of host plant odorants by the yellow peach moth.
GOBP2 蛋白在桃蛀果蛾（Conogethes punctiferalis (Guenée)）中具有独特的功能。在各种鳞翅目物种中已经鉴定出几种通用气味结合蛋白（GOBP），但 GOBP1 和 GOBP2 在识别寄主植物气味方面的功能差异尚不清楚。本研究利用 CRISPR-Cas9 系统评价了 GOBP1 和 GOBP2 在桃蛀果蛾中的功能。结果表明，GOBP2 在桃蛀果蛾的嗅觉机制中起着重要作用。敲除 GOBP1 基因的幼虫对取食的感知下降，但未达到显著水平，而敲除 GOBP2 和 GOBP1/2 基因则产生了巨大差异。另一方面，触角电位图（EAGs）和风洞测试表明，GOBP2 敲除成虫对气味的敏感性下降超过 GOBP1 敲除个体。STRING 数据库文本挖掘的结果引起了我们对蛋白质-蛋白质相互作用研究的关注。在这项研究中，我们首次通过表面等离子体共振（SPR）和等温热力学滴定（ITC）方法证明了 GOBP 和化学感觉蛋白（CSP）之间存在物理相互作用。有趣的是，GOBP1 和 GOBP2 不能相互作用，但它们可以与 CSP 相互作用。相互作用的结果表明，GOBP2 可以与 CSP15、CSP5 和 OBP17 物理相互作用，而 GOBP1 只能与 CSP5 和 CSP10 结合，其结合常数（k）也比 GOBP1 更显著。这些结果强烈表明 GOBP2 在桃蛀果蛾对寄主植物气味的感知中具有重要功能。
