Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
College of Forestry, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
Sci Rep. 2016 Jul 1;6:29067. doi: 10.1038/srep29067.
The yellow peach moth (YPM), Conogethes punctiferalis (Guenée), is a multivoltine insect pest of crops and fruits. Antennal-expressed receptors are important for insects to detect olfactory cues for host finding, mate attraction and oviposition site selection. However, few olfactory related genes were reported in YPM until now. In the present study, we sequenced and characterized the antennal transcriptomes of male and female YPM. In total, 15 putative odorant binding proteins (OBPs), 46 putative odorant receptors (ORs) and 7 putative ionotropic receptors (IRs) were annotated and identified as olfactory-related genes of C. punctiferalis. Further analysis of RT-qPCR revealed that all these olfactory genes are primarily or uniquely expressed in male and female antennae. Among which, 3 OBPs (OBP4, OBP8 and PBP2) and 4 ORs (OR22, OR26, OR44 and OR46) were specially expressed in male antennae, whereas 4 ORs (OR5, OR16, OR25 and OR42) were primarily expressed in female antennae. The predicted protein sequences were compared with homologs in other lepidopteran species and model insects, which showed high sequence homologies between C. punctiferalis and O. furnacalis. Our work allows for further functional studies of pheromone and general odorant detection genes, which might be meaningful targets for pest management.
黄桃潜叶蛾（YPM），Conogethes punctiferalis（Guenée），是一种多化性作物和水果害虫。触角表达的受体对于昆虫探测嗅觉线索以寻找宿主、吸引配偶和选择产卵地点非常重要。然而，到目前为止，在 YPM 中报道的嗅觉相关基因很少。在本研究中，我们对雄性和雌性 YPM 的触角转录组进行了测序和特征分析。总共注释和鉴定了 15 个假定的气味结合蛋白（OBP）、46 个假定的气味受体（OR）和 7 个假定的离子型受体（IR），它们被认为是 C. punctiferalis 的嗅觉相关基因。进一步的 RT-qPCR 分析表明，所有这些嗅觉基因主要或仅在雄性和雌性触角中表达。其中，3 个 OBP（OBP4、OBP8 和 PBP2）和 4 个 OR（OR22、OR26、OR44 和 OR46）在雄性触角中特异性表达，而 4 个 OR（OR5、OR16、OR25 和 OR42）主要在雌性触角中表达。预测的蛋白序列与其他鳞翅目物种和模式昆虫的同源物进行了比较，结果表明 C. punctiferalis 和 O. furnacalis 之间具有高度的序列同源性。我们的工作允许进一步研究信息素和一般气味检测基因的功能，这可能是害虫管理的有意义的目标。
