Centre for Nanosciences and Molecular Medicine, Amrita Vishwa Vidyapeetham, Kochi 682041, Kerala, India.
Department of Microbiology, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi 682041, India.
Int J Biol Macromol. 2021 Dec 15;193(Pt B):1653-1658. doi: 10.1016/j.ijbiomac.2021.11.002. Epub 2021 Nov 3.
Infections on the wound surface are the major problem in restricting the healing process. To reduce the transmission and treat the infection, we have developed 0.05% and 0.1% octenidine dihydrochloride (Ocd) incorporated chitosan (Cs) based flexible bandages. Ocd is extensively used skin antiseptic for its mode of action over a broad spectrum of antimicrobial activity. The prepared antiseptic Cs-Ocd bandage was characterized using Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM). In addition, swelling, degradation, cytocompability, antibacterial, and anti-biofilm property of the developed bandages were studied. This highly porous nature of Cs-Ocd bandage showed enhanced swelling property, slow degradation profile and controlled release of Ocd. The prepared antiseptic bandage exhibited synergistic effect showing good hemostatic potential with Cs, excellent antimicrobial and anti-biofilm activity with Ocd against Staphylococcus aureus (S. aureus) and Candida auris (C. auris). Thus, the developed Cs-Ocd bandage can be used as potential antiseptic bandage for skin infections.
创面感染是限制愈合过程的主要问题。为了减少传播和治疗感染，我们开发了 0.05%和 0.1%盐酸奥替尼啶（Ocd）掺入壳聚糖（Cs）的弹性绷带。Ocd 因其广谱抗菌活性的作用模式而被广泛用作皮肤防腐剂。使用傅里叶变换红外光谱（FT-IR）和扫描电子显微镜（SEM）对制备的防腐剂 Cs-Ocd 绷带进行了表征。此外，还研究了开发绷带的溶胀、降解、细胞相容性、抗菌和抗生物膜性能。Cs-Ocd 绷带具有高度多孔的性质，表现出增强的溶胀性能、缓慢的降解曲线和 Ocd 的控制释放。制备的防腐剂绷带表现出协同作用，Cs 具有良好的止血潜力，Ocd 对金黄色葡萄球菌（S. aureus）和耳念珠菌（C. auris）具有优异的抗菌和抗生物膜活性。因此，开发的 Cs-Ocd 绷带可用作皮肤感染的潜在防腐剂绷带。
