Post Graduate Program in Biophotonics Applied to Health Sciences, University Nove de Julho (UNINOVE), Rua Vergueiro, 239/245, São Paulo, SP, CEP 01504-000, Brazil.
Department of Basic Science in Health, Faculty of Medical Sciences, Federal University of Cuiabá, Cuiabá, Brazil.
Lasers Med Sci. 2022 Apr;37(3):1963-1971. doi: 10.1007/s10103-021-03457-0. Epub 2021 Nov 6.
Corticosteroid-resistant asthma (CRA) is a severe form of disease and clinically important, since patients do not respond to mainstay corticosteroid therapies. Thus, new therapies are needed. However, a big limiting factor in the understanding of CRA is the existence of different immunological and inflammatory phenotypes, a fact that makes it difficult to reproduce experimentally. Photobiomodulation (PBM) emerges as an alternative therapy based on earlier studies. This study aims to evaluate the effect of PBM using infrared light-emitting diode (ILED) on the development of corticosteroid-resistant asthma. Therefore, groups of rats were sensitized and challenged with ovalbumin plus Freund's adjuvant for the induction of CRA, and treated or not with ILED directly in the respiratory tract on the skin (wavelength 810 nm; power 100 mW; density energy 5 J/cm; total energy 15 J; time 150 s). Our experimental model was capable to induce neutrophilic asthma. Besides that, the corticosteroid treatment did not reverse the lung cell migration as well as the levels of leukotriene B4, and interleukins 17 and 6. The treatment with ILED reduced the lung cell migration; myeloperoxidase activity; mast cell degranulation; and the levels of leukotriene B4, thromboxane B2, prostaglandin E2, tumoral necrosis factor alpha, and interleukins 17 and 6. Still, ILED increased the level of interleukin 10. In conclusion, we showed promisor effects of ILED when irradiated directly in the respiratory tract as adjuvant treatment of corticosteroid-resistant asthma.
皮质类固醇抵抗性哮喘（CRA）是一种严重的疾病形式，具有重要的临床意义，因为患者对主要的皮质类固醇治疗没有反应。因此，需要新的治疗方法。然而，理解 CRA 的一个主要限制因素是存在不同的免疫和炎症表型，这使得难以在实验中重现。光生物调节（PBM）作为一种基于早期研究的替代治疗方法出现。本研究旨在评估使用红外发光二极管（ILED）进行 PBM 对皮质类固醇抵抗性哮喘发展的影响。因此，将大鼠分为几组，用卵清蛋白和福氏佐剂进行敏化和攻击，以诱导 CRA，并在皮肤（波长 810nm；功率 100mW；能量密度 5J/cm；总能量 15J；时间 150s）上直接在呼吸道内用 ILED 治疗或不治疗。我们的实验模型能够诱导中性粒细胞性哮喘。此外，皮质类固醇治疗不能逆转肺细胞迁移以及白三烯 B4 和白细胞介素 17 和 6 的水平。ILED 治疗可减少肺细胞迁移；髓过氧化物酶活性；肥大细胞脱颗粒；白三烯 B4、血栓素 B2、前列腺素 E2、肿瘤坏死因子α 和白细胞介素 17 和 6 的水平。然而，ILED 增加了白细胞介素 10 的水平。总之，我们展示了 ILED 在直接照射呼吸道作为皮质类固醇抵抗性哮喘辅助治疗时的有希望的效果。
