Department of Dermatology and Venereology, First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Kunming, 650032, Yunnan, China.
College of Chinese Materia Medica, Yunnan University of Chinese Medicine, 1076 Yuhua Road, Chenggong, Kunming, 650500, Yunnan, China.
Mycopathologia. 2022 Feb;187(1):53-64. doi: 10.1007/s11046-021-00596-4. Epub 2021 Nov 7.
Talaromycosis (penicilliosis) caused by Talaromyces marneffei is one of the most important opportunistic infection diseases in tropical countries of South and Southeast Asia. Most infections occurred in individuals with human immunodeficiency virus (HIV) and the primarily reason for the increase in the number of the cases is HIV pandemic. The pathogenesis of T. marneffei infection is unclear. There is still no ideal animal model for studying talaromycosis. In this study, we developed a stable, safe and maneuverable murine model that mimics human T. marneffei disseminated infection using T. marneffei yeast intraperitoneal injected to BALB/c nude mice. We successfully observed symptoms similar to those seen in clinical patients in this murine model, including skin lesions, hepatosplenomegaly, pulmonary infection and mesenteric lesions. We further studied the pathological changes of various tissues and organs in the infected animals to help better understand the severity of the infection. This model may provide a good tool for studying disseminated infection induced by T. marneffei.
马尔尼菲青霉病（马尔尼菲篮状菌病）是由马尔尼菲篮状菌引起的，是南亚和东南亚热带国家最重要的机会性感染疾病之一。大多数感染发生在人类免疫缺陷病毒（HIV）感染者中，病例数量增加的主要原因是 HIV 流行。马尔尼菲篮状菌感染的发病机制尚不清楚。目前仍然没有理想的动物模型来研究马尔尼菲篮状菌病。在这项研究中，我们使用马尔尼菲篮状菌酵母腹腔注射 BALB/c 裸鼠，建立了一种稳定、安全且易于操作的模拟人类马尔尼菲篮状菌播散性感染的小鼠模型。我们成功地在该小鼠模型中观察到了类似于临床患者的症状，包括皮肤损伤、肝脾肿大、肺部感染和肠系膜病变。我们进一步研究了感染动物各组织和器官的病理变化，以帮助更好地了解感染的严重程度。该模型可能为研究马尔尼菲篮状菌引起的播散性感染提供了一个很好的工具。
