Feng Jiao, Chen Zhiwen, He Liya, Xiao Xing, Chen Chunmei, Chu Jieming, Mylonakis Eleftherios, Xi Liyan
Department of Dermatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, USA.
Mycobiology. 2019 Jun 17;47(2):207-216. doi: 10.1080/12298093.2019.1620975. eCollection 2019.
is the only dimorphic species in its genus and causes a fatal systemic mycosis named talaromycosis. Our previous study indicated that knockdown of gene (encodes isocitrate lyase of glyoxylate bypass) of by RNA interference approach attenuated the virulence of , while the virulence of the knockout strains was not studied. In this study, -zebrafish infection model was successfully established through hindbrain microinjection with different amounts of yeast cells. After co-incubated at 28°C, the increasing inoculum doses result in greater larval mortality; and hyphae generation might be one virulence factor involved in zebrafish infection. Moreover, the results demonstrated that the virulence of the was significantly attenuated in this Zebrafish infection model.
它是其属中唯一的双态物种，可引起一种名为塔拉霉病的致命系统性真菌病。我们之前的研究表明，通过RNA干扰方法敲低该基因（编码乙醛酸旁路的异柠檬酸裂解酶）可减弱其毒力，而该基因敲除菌株的毒力尚未研究。在本研究中，通过后脑微量注射不同数量的酵母细胞成功建立了斑马鱼感染模型。在28°C共孵育后，接种剂量增加导致幼虫死亡率更高；并且菌丝生成可能是参与斑马鱼感染的一个毒力因素。此外，结果表明在该斑马鱼感染模型中其毒力显著减弱。
