等离子体增强铜掺杂 TiO2 富氧空位的鲁米诺电致化学发光用于碱性磷酸酶的高灵敏检测。
Plasmon-Boosted Cu-Doped TiO Oxygen Vacancy-Rich Luminol Electrochemiluminescence for Highly Sensitive Detection of Alkaline Phosphatase.
机构信息
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, P.R. China.
University of Science and Technology of China, No. 96 JinZhai Road, Hefei, Anhui 230026, P.R. China.
出版信息
Anal Chem. 2021 Nov 16;93(45):15183-15191. doi: 10.1021/acs.analchem.1c03842. Epub 2021 Nov 7.
In this study, an effective oxygen vacancy (O)-involved luminol-dissolved oxygen (O) electrochemiluminescence (luminol-DO ECL) system was developed and exploited for ECL sensing applications through significant plasmon enhancement of the O-involved weak luminol-DO ECL signals by the combined use of Cu-doped TiO oxygen vacancy and a Au@SiO nanomembrane. The results disclosed that the ECL response of the corresponding system could be synergistically boosted, and the plausible underlying mechanism has been discussed. Furthermore, for the first time, the developed system has been successfully applied for the highly sensitive detection of alkaline phosphatase with a low limit of detection of 0.005 U/L, with an excellent linear range from 0.005 to 10 U/L, as well as good stability and reproducibility.
在这项研究中，我们开发了一种有效的氧空位（O）参与的鲁米诺溶解氧（O）电化学发光（鲁米诺-DO ECL）系统，并通过使用 Cu 掺杂 TiO 氧空位和 Au@SiO 纳米膜，显著增强了 O 参与的弱鲁米诺-DO ECL 信号的等离子体增强作用，从而将其应用于 ECL 传感。结果表明，相应体系的 ECL 响应可以协同增强，并且讨论了可能的潜在机制。此外，该系统还首次被成功应用于碱性磷酸酶的高灵敏度检测，检测限低至 0.005 U/L，线性范围从 0.005 到 10 U/L，具有良好的稳定性和重现性。
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