需氧氧化催化剂乙酰丙酮氧钒增强鲁米诺化学发光用于碱性磷酸酶和抗坏血酸的灵敏检测。
Aerobic oxidation catalyst vanadyl acetylacetonate boosts luminol chemiluminescence for sensitive detection of alkaline phosphatase and ascorbic acid.
作者信息
Zhang Wei, Chen Yequan, Ji Kaixiang, Dong Zhiyong, Abdussalam Abubakar, Zholudov Yuriy T, Gao Wenyue, Tian Yu, Xu Guobao
机构信息
School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026, Anhui, China.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, Jilin, China.
出版信息
Anal Bioanal Chem. 2025 May;417(13):2879-2887. doi: 10.1007/s00216-025-05827-x. Epub 2025 Mar 17.
Vanadyl acetylacetonate, VO(acac), is a well-known environmentally friendly catalyst for aerobic oxidations. It has been exploited as an efficient co-reactant of luminol chemiluminescence (CL) in aqueous media for the first time. The luminol-VO(acac) system generates an intense CL and ascorbic acid can quench its CL remarkably. Based on the strong quenching effect of ascorbic acid, the luminol-VO(acac) CL was used in the quantification of clinically important ascorbic acid and alkaline phosphatase (ALP) by enzymatic conversion of L-ascorbic acid 2-phosphate into ascorbic acid. The linear ranges for detecting ascorbic acid and alkaline phosphatase are 1 to 100 μM and 0.005 to 200 U/L, respectively. The limits of detection of ascorbic acid and alkaline phosphatase are 0.5 μM and 0.005 U/L, respectively. The proposed CL method has shown high selectivity for the ALP among the tested biomolecules and metal ions. It also shows good recoveries in human serum samples.
乙酰丙酮氧钒（VO(acac)）是一种著名的用于需氧氧化的环境友好型催化剂。它首次被用作鲁米诺化学发光（CL）在水介质中的有效共反应物。鲁米诺 - VO(acac)体系产生强烈的化学发光，而抗坏血酸能显著淬灭其化学发光。基于抗坏血酸的强淬灭作用，鲁米诺 - VO(acac)化学发光被用于通过将L - 抗坏血酸2 - 磷酸酶促转化为抗坏血酸来定量临床重要的抗坏血酸和碱性磷酸酶（ALP）。检测抗坏血酸和碱性磷酸酶的线性范围分别为1至100 μM和0.005至200 U/L。抗坏血酸和碱性磷酸酶的检测限分别为0.5 μM和0.005 U/L。所提出的化学发光方法在测试的生物分子和金属离子中对碱性磷酸酶具有高选择性。它在人血清样品中也显示出良好的回收率。
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