Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010000, China.
Inner Mongolia-CABI Joint Laboratory for Grassland Protection and Sustainable Utilization, Chinese Academy of Agricultural Sciences, Hohhot 010000, China.
Int J Mol Sci. 2024 Nov 18;25(22):12360. doi: 10.3390/ijms252212360.
Lepidopteran insects are a major threat to global agriculture, causing significant crop losses and economic damage. Traditional pest control methods are becoming less effective due to the rapid evolution of insecticide resistance. This study explores the current status and genomic characteristics of 1315 Lepidopteran records, alongside an overview of relevant research, utilizing advanced functional genomics techniques, including RNA-seq and CRISPR/Cas9 gene-editing technologies to uncover the molecular mechanisms underlying insecticide resistance. Our genomic analysis revealed significant variability in genome size, assembly quality, and chromosome number, which may influence species' biology and resistance mechanisms. We identified key resistance-associated genes and pathways, including detoxification and metabolic pathways, which help these insects evade chemical control. By employing CRISPR/Cas9 gene-editing techniques, we directly manipulated resistance-associated genes to confirm their roles in resistance, demonstrating their potential for targeted interventions in pest management. These findings emphasize the value of integrating genomic data into the development of effective and sustainable pest control strategies, reducing reliance on chemical insecticides and promoting environmentally friendly integrated pest management (IPM) approaches. Our study highlights the critical role of functional genomics in IPM and its potential to provide long-term solutions to the growing challenge of Lepidopteran resistance.
鳞翅目昆虫对全球农业构成重大威胁，导致重大作物损失和经济损失。由于杀虫剂抗性的快速进化，传统的害虫防治方法变得越来越不有效。本研究利用先进的功能基因组学技术，包括 RNA-seq 和 CRISPR/Cas9 基因编辑技术，探索了 1315 个鳞翅目记录的现状和基因组特征，以及相关研究的概述，以揭示昆虫抗药性的分子机制。我们的基因组分析显示，基因组大小、组装质量和染色体数存在显著差异，这可能影响物种的生物学和抗性机制。我们确定了关键的抗性相关基因和途径，包括解毒和代谢途径，这些途径帮助这些昆虫逃避化学控制。通过使用 CRISPR/Cas9 基因编辑技术，我们直接操纵抗性相关基因，以确认它们在抗性中的作用，展示了它们在害虫管理中的靶向干预的潜力。这些发现强调了将基因组数据整合到有效的和可持续的害虫防治策略的开发中的价值，减少对化学杀虫剂的依赖，并促进环保的综合害虫管理（IPM）方法。我们的研究强调了功能基因组学在 IPM 中的关键作用及其为应对日益严重的鳞翅目抗性挑战提供长期解决方案的潜力。
