Ismaiel Mostafa M S, El-Ayouty Yassin M, Fathey Hoda A
Department of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig, 44519 Egypt.
Physiol Mol Biol Plants. 2021 Oct;27(10):2151-2163. doi: 10.1007/s12298-021-01077-0. Epub 2021 Sep 30.
Two strains of the halophilic alga , a wild type (WT) and a transgenic strain () containing an exogenous phytoene synthase gene (), were used to investigate the growth, carotenoid accumulation, and carotenoid antioxidant properties under nitrogen starvation, cobalt and biochar treatments. had higher carotenoid content (1.8 times) compared to the WT. The applied stressors stimulated the carotenoid content of both WT and especially. The carotenoids were assayed for the potential antioxidant activities by five different assays. Generally, the antioxidant activities of carotenoids were superior to that of WT. The biochar and nitrogen treatments generally enhanced the antioxidant activities of the carotenoids, whereas cobalt came third in this respect. The transgenic algal strain has both high carotenoids content and antioxidant properties which enhanced under the relatively lower concentrations of the applied stressors. The results have shown to lead to an accurate application of the transgenic alga as a source of potent antioxidant compounds.
The online version contains supplementary material available at 10.1007/s12298-021-01077-0.
使用两株嗜盐藻，一株野生型（WT）和一株含有外源八氢番茄红素合成酶基因（）的转基因藻株（），研究在氮饥饿、钴和生物炭处理下的生长、类胡萝卜素积累及类胡萝卜素抗氧化特性。与野生型相比，（）的类胡萝卜素含量更高（1.8倍）。所施加的应激源尤其刺激了野生型和（）的类胡萝卜素含量。通过五种不同的测定方法检测类胡萝卜素的潜在抗氧化活性。一般来说，（）类胡萝卜素的抗氧化活性优于野生型。生物炭和氮处理通常增强了类胡萝卜素的抗氧化活性，而钴在这方面排第三。（）转基因藻株具有高类胡萝卜素含量和抗氧化特性，在相对较低浓度的所施加应激源下会增强。结果表明可准确将转基因藻用作强效抗氧化化合物的来源。
在线版本包含可在10.1007/s12298-021-01077-0获取的补充材料。
