Cheng Jie, Zhou Songsong, Yang Kun, Yu Hongyang, Chen Rongrong, Zeng Liming, Li Hua, Wang Yihua, Song Jianbo
College of Biological Sciences and Engineering, Jiangxi Agricultural University, Nanchang, 330045 People's Republic of China.
Camphor Engineering Technology Research Center for State Forestry Administration, Jiangxi Academy of Forestry, Nanchang, 330032 People's Republic of China.
Physiol Mol Biol Plants. 2021 Oct;27(10):2283-2296. doi: 10.1007/s12298-021-01087-y. Epub 2021 Oct 13.
RNA helicase catalyzes the denaturation of DNA or the unwinding of double-stranded RNA. It is vital to RNA splicing, transport, editing, degradation and the initiation of protein translation. However, the function of RNA helicase in has rarely been reported. In this study, 170 putative RNA helicase genes were identified in the genome, and classified into three subfamilies based on the presence of either a DEAD-box (52 genes), DEAH-box (38 genes), or DExD/H-box (80 genes) in their coding regions. Additionally, conserved helicase_C domains and other functional domains (e.g., the HA2, DUF, and ZnF domains) were also present in these genes. Chromosomal mapping and synteny analyses showed that there were tandem and segment duplications of RNA helicase genes. Furthermore, transcriptome and real-time PCR analysis showed that the expression of 35 RNA helicase genes was affected by abiotic stress. To be specific, 17, 12 and 19 genes were regulated by salt, drought and cold stress, respectively. It is worth noting that and responded to all three types of stress. These results provide valuable information for understanding the RNA helicase genes in and their abiotic stress-related functions.
The online version contains supplementary material available at 10.1007/s12298-021-01087-y.
RNA解旋酶催化DNA变性或双链RNA解旋。它对RNA剪接、转运、编辑、降解及蛋白质翻译起始至关重要。然而，RNA解旋酶在[具体物种]中的功能鲜有报道。本研究在[具体物种]基因组中鉴定出170个推定的RNA解旋酶基因，并根据其编码区中是否存在DEAD-box（52个基因）、DEAH-box（38个基因）或DExD/H-box（80个基因）分为三个亚家族。此外，这些基因中还存在保守的解旋酶_C结构域和其他功能结构域（如HA2、DUF和ZnF结构域）。染色体定位和共线性分析表明，RNA解旋酶基因存在串联和片段重复。此外，转录组和实时PCR分析表明，35个RNA解旋酶基因的表达受非生物胁迫影响。具体而言，分别有17、12和19个基因受盐、干旱和冷胁迫调控。值得注意的是，[具体基因1]和[具体基因2]对所有三种胁迫均有响应。这些结果为理解[具体物种]中的RNA解旋酶基因及其与非生物胁迫相关的功能提供了有价值的信息。
在线版本包含可在10.1007/s12298-021-01087-y获取的补充材料。
