体外D-丝氨酸脱氨酶合成的阳性调控
Positive control of D-serine deaminase synthesis in vitro.
作者信息
Heincz M C, Kelker N E, McFall E
出版信息
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1695-9. doi: 10.1073/pnas.75.4.1695.
Abstract
Efficient constitutive synthesis of D-serine deaminase [D-serine hydro-lyase (deaminating); EC 4.2.1.14] is obtained in vitro by using a slightly modified Zubay system programmed with dsdO6 dsdA+DNA. Synthesis from a dsdO+ dsdA+ template requires active dsdC gene product and 3':5'-cyclic AMP. In vitro synthesis of dsdC product is obtained with a dsdC+ dsdO+ dsdA+ or a dsdCc dsdO+ dsdA+ template; this synthesis is thermosensitive and can be uncoupled from D-serine deaminase synthesis by temperature shift.
摘要
通过使用用dsdO6 dsdA + DNA编程的稍加修改的祖拜系统，可在体外高效组成型合成D - 丝氨酸脱氨酶[D - 丝氨酸水解酶（脱氨基）；EC 4.2.1.14]。从dsdO + dsdA +模板合成需要活性dsdC基因产物和3':5'-环磷酸腺苷。用dsdC + dsdO + dsdA +或dsdCc dsdO + dsdA +模板可在体外合成dsdC产物；这种合成是温度敏感的，并且可以通过温度变化与D - 丝氨酸脱氨酶合成解偶联。
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