Bornstein-Forst S M, McFall E, Palchaudhuri S
J Bacteriol. 1987 Mar;169(3):1056-60. doi: 10.1128/jb.169.3.1056-1060.1987.
The D-serine deaminase structural (dsdA) and regulatory (dsdC) genes are transcribed with opposite polarity from an intergenic region comprising more than 600 base pairs. The order of genes in the dsd region is supN-dsdA-dsdC-aroC---his. The DNA sequence of the intergenic region has been slightly revised from a previously published version (E. McFall and L. Runkel, J. Bacteriol. 154:1508-1512, 1983). The dsdA gene is preceded by a long open reading frame. The dsdA in vivo transcription start sites for the wild type (base pair +1) and for three phenotypically distinct promoter constitutive mutants were determined by the S1 nuclease method. They are identical and are located about 81 base pairs upstream of the translation start site. D-Serine deaminase regulation is normal in rho mutants. Possible mechanisms for dsdA activation are discussed.
D-丝氨酸脱氨酶结构基因（dsdA）和调控基因（dsdC）从一个包含600多个碱基对的基因间区域以相反的极性转录。dsd区域中的基因顺序为supN-dsdA-dsdC-aroC---his。基因间区域的DNA序列与先前发表的版本（E. 麦克法尔和L. 伦克尔，《细菌学杂志》154:1508 - 1512，1983年）略有修订。dsdA基因之前有一个长开放阅读框。通过S1核酸酶法确定了野生型（碱基对 +1）和三个表型不同的启动子组成型突变体的dsdA体内转录起始位点。它们是相同的，位于翻译起始位点上游约81个碱基对处。在rho突变体中，D-丝氨酸脱氨酶的调控是正常的。讨论了dsdA激活的可能机制。
