Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Front Immunol. 2021 Oct 20;12:766740. doi: 10.3389/fimmu.2021.766740. eCollection 2021.
Duck viral hepatitis (DVH) is an acute, highly lethal infectious disease of ducklings that causes huge losses in the duck industry. Duck hepatitis A virus genotype 3 (DHAV-3) has been one of the most prevalent DVH pathogen in the Asian duck industry in recent years. Here, we investigated the genetic basis of the resistance and susceptibility of ducks to DVH by comparing the genomes and transcriptomes of a resistant Pekin duck flock (Z8) and a susceptible Pekin duck flock (SZ7). Our comparative genomic and transcriptomic analyses suggested that showed a strong signal of association with DVH susceptibility in ducks. Then, we found that showed a significant expression difference between the livers of susceptible and resistant individuals after infection with DHAV-3, with higher expression in the SZ7 flock. Furthermore, suppression and overexpression experiments showed that the number of DHAV-3 genomic copies in primary duck hepatocytes was influenced by the expression level of . In addition, RNAscope analysis showed that the localization of and DHAV-3 in liver cells was consistent. Altogether, our data suggested that was likely associated with DHAV-3 susceptibility in ducks, which provides a target for future investigations of the pathogenesis of DVH.
鸭病毒性肝炎（DVH）是一种急性、高致死性的鸭传染病，给养鸭业造成了巨大损失。近年来，鸭甲型肝炎病毒 3 型（DHAV-3）已成为亚洲养鸭业中最流行的 DVH 病原体之一。在这里，我们通过比较抗鸭病毒性肝炎的北京鸭种群（Z8）和易感北京鸭种群（SZ7）的基因组和转录组，研究了鸭对 DVH 的抗性和易感性的遗传基础。我们的比较基因组和转录组分析表明，在鸭子中，与 DVH 易感性强烈相关。然后，我们发现，在感染 DHAV-3 后，在易感和抗性个体的肝脏中，显示出显著的表达差异，在 SZ7 群体中表达水平更高。此外，抑制和过表达实验表明，DHAV-3 基因组拷贝数在原代鸭肝细胞中的数量受 表达水平的影响。此外，RNAscope 分析表明，和 DHAV-3 在肝细胞中的定位是一致的。总之，我们的数据表明，可能与鸭对 DHAV-3 的易感性有关，这为今后研究 DVH 的发病机制提供了一个目标。
