Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture and Rural Affairs), Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China.
Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Front Cell Infect Microbiol. 2022 Jul 8;12:927840. doi: 10.3389/fcimb.2022.927840. eCollection 2022.
is a highly contagious pathogen causing porcine enzootic pneumonia, which elicits prolonged inflammatory response modulated by pattern recognition receptors (PRRs). Although significant advances have been achieved in understanding the Toll-Like receptors that recognize , the role of nucleotide-binding oligomerization domain 1 (NOD1) in infected cells remains poorly understood. This study revealed that activates the NOD1-RIP2 pathway and is co-localized with host NOD1 during infection. siRNA knockdown of NOD1 significantly impaired the TRIF and MYD88 pathway and blocked the activation of TNF-α. In contrast, NOD1 overexpression significantly suppressed proliferation. Furthermore, we for the first time investigated the interaction between mhp390 and NOD1 receptor, and the results suggested that mhp390 and NOD1 are possibly involved in the recognition of These findings may improve our understanding of the interaction between PRRs and and the function of NOD1 in host defense against infection.
是一种高度传染性病原体，可引起猪地方性肺炎，引发由模式识别受体（PRRs）调节的长期炎症反应。尽管在理解识别的 Toll 样受体方面已经取得了重大进展，但宿主细胞中核苷酸结合寡聚化结构域 1（NOD1）在 感染中的作用仍知之甚少。本研究表明， 激活 NOD1-RIP2 途径，并在感染过程中与宿主 NOD1 共定位。NOD1 的 siRNA 敲低显著削弱了 TRIF 和 MYD88 途径，并阻断了 TNF-α的激活。相反，NOD1 的过表达显著抑制了 的增殖。此外，我们首次研究了 mhp390 与 NOD1 受体之间的相互作用，结果表明 mhp390 和 NOD1 可能参与了 的识别。这些发现可能有助于我们理解 PRRs 与 之间的相互作用以及 NOD1 在宿主防御 感染中的功能。
