State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Department of Animal Resource & Science, Dankook University, Cheonan, Choongnam 330-714, Republic of Korea.
J Immunol Res. 2021 Oct 27;2021:2549541. doi: 10.1155/2021/2549541. eCollection 2021.
(CP) is the principal pathogenic bacterium of chicken necrotic enteritis (NE), which causes substantial economic losses in poultry worldwide. Although probiotics are known to provide multiple benefits, little is known about the potential effects of (. ) application in preventing CP-induced necrotic enteritis. In this study, 450 male Arbor Acres broilers were divided into 5 experimental treatments: A: basal diet (control group); B: basal diet and CP challenge (model group); C: CP challenge+10 mg/kg enramycin (positive control group); D: CP challenge+4 × 10 CFU/kg of feed . PB6 (PB6 low-dosage group); and E: CP challenge+6 × 10 CFU/kg of feed . PB6 (PB6 high-dosage group). There were 6 replicate pens per treatment with 15 broilers per pen. The present research examined the effect of PB6 (. PB6) on growth performance, mRNA expression of intestinal cytokines and tight junctions, and gut flora composition in broilers challenged with CP. The entire experiment was divided into two phases: the non-CP challenge phase (d0-18) and the CP challenge phase (d18-26). PB6 did not increase the growth performance during the first stage, but the PB6 high-dosage group was found to have larger body weight gain and ADFI during the CP challenge stage. Feed supplementation with PB6 reduced the lesion score of challenged chicks, with increased tight junction-related gene expression ( and -) and decreased - expression compared with CP-infected birds. A decrease in the abundance of , s, and was observed after CP infection ( < 0.05), while supplementation with PB6 restored the ileal microbial composition. In conclusion, administration of . PB6 improved growth performance, enhanced intestinal barrier function, and mitigated intestinal inflammation/lesions, which might be due to its restoring effects on the ileal microbial composition in CP-challenged broilers.
（CP）是鸡坏死性肠炎（NE）的主要病原菌，它在世界范围内给家禽养殖业造成了巨大的经济损失。虽然益生菌被认为具有多种益处，但人们对其应用于预防 CP 诱导的坏死性肠炎的潜在影响知之甚少。在本研究中，将 450 只雄性 Arbor Acres 肉鸡分为 5 个实验组：A：基础日粮（对照组）；B：基础日粮和 CP 攻毒（模型组）；C：CP 攻毒+10mg/kg 恩拉霉素（阳性对照组）；D：CP 攻毒+4×10CFU/kg 饲料. PB6（PB6 低剂量组）；E：CP 攻毒+6×10CFU/kg 饲料. PB6（PB6 高剂量组）。每个处理组有 6 个重复笼，每个笼有 15 只肉鸡。本研究考察了. PB6（PB6）对 CP 攻毒肉鸡生长性能、肠道细胞因子和紧密连接相关基因表达以及肠道菌群组成的影响。整个实验分为两个阶段：非 CP 攻毒阶段（d0-18）和 CP 攻毒阶段（d18-26）。PB6 在第一阶段没有增加生长性能，但 PB6 高剂量组在 CP 攻毒阶段的体重增加和 ADFI 较大。与 CP 感染的雏鸡相比，补充 PB6 降低了攻毒雏鸡的病变评分，增加了与紧密连接相关的基因表达（和 -），降低了 - 的表达。CP 感染后观察到 、s 和 的丰度降低（<0.05），而补充 PB6 恢复了回肠微生物组成。总之，. PB6 的给药改善了生长性能，增强了肠道屏障功能，减轻了肠道炎症/病变，这可能是由于其对 CP 攻毒肉鸡回肠微生物组成的恢复作用。
