Zhong Yifan, Zhang Meiting, Xu Haocheng, Yu Xiaorong, Hu Yashi, Xu Yangyi, Xiao Xiao, Yang Caimei
College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang Agricultural and Forestry University, Hangzhou 311300, China.
Animals (Basel). 2025 May 13;15(10):1409. doi: 10.3390/ani15101409.
() is a probiotic known for its ability to enhance host resistance against pathogenic infections. This study aimed to evaluate the protective effects and underlying mechanisms of in a mouse model challenged with (). C57BL/6J mice were pretreated with for 21 days before oral infection with . The probiotic administration significantly prevented infection-induced weight loss and immune organ enlargement. Serum cytokine analysis revealed that increased anti-inflammatory IL-4 and IL-10 levels while reducing pro-inflammatory IL-1β, IL-6, and TNF-α levels. Histological analysis showed that preserved intestinal morphology and inhibited epithelial cell apoptosis. Moreover, the probiotic mitigated the infection-induced decline in volatile fatty acid (VFA) production. 16S rRNA gene sequencing revealed that reshaped the cecal microbiota, characterized by the increased abundance of , , and , and reduced abundance of . Untargeted metabolomic profiling identified differential metabolites-including D-glucono-1,5-lactone, D-erythrose 4-phosphate, and D-sedoheptulose 7-phosphate-enriched in the pentose phosphate pathway, suggesting a regulatory role in redox homeostasis and host response. Collectively, these results indicate that exerts protective effects against infection by modulating inflammation, apoptosis, microbial composition, and metabolic pathways. This work provides new insights into the application of as a functional microbial feed additive in livestock disease prevention.
（）是一种益生菌，以其增强宿主对病原体感染的抵抗力的能力而闻名。本研究旨在评估（）在感染（）的小鼠模型中的保护作用及潜在机制。C57BL/6J小鼠在经口感染（）前用（）预处理21天。益生菌给药显著预防了感染诱导的体重减轻和免疫器官肿大。血清细胞因子分析显示，（）增加了抗炎性白细胞介素-4和白细胞介素-10水平，同时降低了促炎性白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α水平。组织学分析表明，（）保留了肠道形态并抑制了上皮细胞凋亡。此外，该益生菌减轻了感染诱导的挥发性脂肪酸（VFA）产生的下降。16S rRNA基因测序显示，（）重塑了盲肠微生物群，其特征是（）、（）和（）丰度增加，（）丰度降低。非靶向代谢组学分析确定了在磷酸戊糖途径中富集的差异代谢物，包括D-葡萄糖酸-1,5-内酯、D-赤藓糖4-磷酸和D-景天庚酮糖7-磷酸，表明其在氧化还原稳态和宿主反应中具有调节作用。总体而言，这些结果表明，（）通过调节炎症、细胞凋亡、微生物组成和代谢途径对（）感染发挥保护作用。这项工作为（）作为功能性微生物饲料添加剂在预防家畜疾病中的应用提供了新的见解。
