• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

转录失活逆转录病毒载体的基因表达

Gene expression from transcriptionally disabled retroviral vectors.

作者信息

Yee J K, Moores J C, Jolly D J, Wolff J A, Respess J G, Friedmann T

出版信息

Proc Natl Acad Sci U S A. 1987 Aug;84(15):5197-201. doi: 10.1073/pnas.84.15.5197.

DOI:10.1073/pnas.84.15.5197
PMID:3474647
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC298821/
Abstract

Retroviral vectors are used for the efficient transfer of foreign genes into mammalian cells. We report here the construction of murine retrovirus-based vectors carrying the full-length cDNA for human hypoxanthine phosphoribosyltransferase (HPRT; EC 2.4.2.8) and from which the enhancer sequences, the "CAAT box," and the "TATA box" in the long terminal repeats (LTRs) have been deleted. After infection of HPRT-deficient rat cells by the vectors, transcriptional activity from the 5' LTR was undetectable and expression of the HPRT cDNA was dependent on an internal promoter. Removal of the LTR regulatory elements increased HPRT gene expression from an internal promoter, indicating interference between the two sets of transcriptional signals. Such disabled vectors may reduce the likelihood of undesirable genetic changes through insertional mutagenesis in cells infected with retroviral vectors.

摘要

逆转录病毒载体被用于将外源基因高效导入哺乳动物细胞。我们在此报告基于鼠逆转录病毒构建的载体，其携带人次黄嘌呤磷酸核糖转移酶（HPRT；EC 2.4.2.8）的全长cDNA，并且长末端重复序列（LTRs）中的增强子序列、“CAAT盒”和“TATA盒”已被删除。用这些载体感染HPRT缺陷型大鼠细胞后，无法检测到来自5' LTR的转录活性，并且HPRT cDNA的表达依赖于一个内部启动子。去除LTR调控元件可增加来自内部启动子的HPRT基因表达，这表明两组转录信号之间存在干扰。这种失活载体可能会降低在感染逆转录病毒载体的细胞中通过插入诱变产生不良基因变化的可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39dc/298821/c889bd0f073c/pnas00330-0121-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39dc/298821/6189ffea090e/pnas00330-0121-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39dc/298821/8e5c52b28462/pnas00330-0121-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39dc/298821/c889bd0f073c/pnas00330-0121-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39dc/298821/6189ffea090e/pnas00330-0121-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39dc/298821/8e5c52b28462/pnas00330-0121-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39dc/298821/c889bd0f073c/pnas00330-0121-c.jpg

相似文献

1
Gene expression from transcriptionally disabled retroviral vectors.转录失活逆转录病毒载体的基因表达
Proc Natl Acad Sci U S A. 1987 Aug;84(15):5197-201. doi: 10.1073/pnas.84.15.5197.
2
Genomic position influences spontaneous mutagenesis of an integrated retroviral vector containing the hprt cDNA as target for mutagenesis.基因组位置影响以hprt互补DNA作为诱变靶点的整合逆转录病毒载体的自发诱变。
Hum Mol Genet. 1993 Feb;2(2):173-82. doi: 10.1093/hmg/2.2.173.
3
A transmissible retrovirus expressing human hypoxanthine phosphoribosyltransferase (HPRT): gene transfer into cells obtained from humans deficient in HPRT.一种表达人次黄嘌呤磷酸核糖转移酶（HPRT）的可传播逆转录病毒：基因转移至来自缺乏HPRT的人类细胞中。
Proc Natl Acad Sci U S A. 1983 Aug;80(15):4709-13. doi: 10.1073/pnas.80.15.4709.
4
Self-inactivating retroviral vectors designed for transfer of whole genes into mammalian cells.为将完整基因导入哺乳动物细胞而设计的自失活逆转录病毒载体。
Proc Natl Acad Sci U S A. 1986 May;83(10):3194-8. doi: 10.1073/pnas.83.10.3194.
5
Mutation induction by UV light in retroviral hprt cDNA integrated at various chromosomal positions in repair-deficient hamster cells.紫外线在修复缺陷型仓鼠细胞中整合于不同染色体位置的逆转录病毒hprt cDNA中诱导突变。
Mutagenesis. 1993 Sep;8(5):399-406. doi: 10.1093/mutage/8.5.399.
6
Construction of a defective retrovirus containing the human hypoxanthine phosphoribosyltransferase cDNA and its expression in cultured cells and mouse bone marrow.一种含有人类次黄嘌呤磷酸核糖转移酶互补脱氧核糖核酸的缺陷型逆转录病毒的构建及其在培养细胞和小鼠骨髓中的表达。
Mol Cell Biol. 1987 Feb;7(2):854-63. doi: 10.1128/mcb.7.2.854-863.1987.
7
Infectious and selectable retrovirus containing an inducible rat growth hormone minigene.含有可诱导大鼠生长激素小基因的传染性和可选择逆转录病毒。
Science. 1984 Sep 7;225(4666):993-8. doi: 10.1126/science.6089340.
8
Evidence that a major class of mouse endogenous long terminal repeats (LTRs) resulted from recombination between exogenous retroviral LTRs and similar LTR-like elements (LTR-IS).有证据表明，一类主要的小鼠内源性长末端重复序列（LTRs）是由外源性逆转录病毒LTRs与类似的LTR样元件（LTR-IS）之间的重组产生的。
Proc Natl Acad Sci U S A. 1984 Nov;81(21):6696-700. doi: 10.1073/pnas.81.21.6696.
9
Liver-directed gene therapy: a retroviral vector with a complete LTR and the ApoE enhancer-alpha 1-antitrypsin promoter dramatically increases expression of human alpha 1-antitrypsin in vivo.肝脏靶向基因治疗：一种带有完整长末端重复序列（LTR）和载脂蛋白E（ApoE）增强子-α1-抗胰蛋白酶启动子的逆转录病毒载体可显著提高人α1-抗胰蛋白酶在体内的表达。
Hum Gene Ther. 1996 Mar 20;7(5):637-45. doi: 10.1089/hum.1996.7.5-637.
10
Nucleotide sequence analysis of avian retroviruses: structural similarities with transposable elements.禽逆转录病毒的核苷酸序列分析：与转座元件的结构相似性
Fed Proc. 1982 Aug;41(10):2659-61.

引用本文的文献

1
The design of retroviral vectors used in the CAR-T products, risk management, and future perspective.嵌合抗原受体T细胞（CAR-T）产品中使用的逆转录病毒载体设计、风险管理及未来展望。
MedComm (2020). 2025 Jan 24;6(2):e70067. doi: 10.1002/mco2.70067. eCollection 2025 Feb.
2
Promoter choice: Who should drive the CAR in T cells?启动子选择：谁应该在 T 细胞中驱动 CAR？
PLoS One. 2020 Jul 24;15(7):e0232915. doi: 10.1371/journal.pone.0232915. eCollection 2020.
3
Simple viral/minimal piggyBac hybrid vectors for stable production of self-inactivating gamma-retroviruses.

本文引用的文献

1
Human metallothionein genes--primary structure of the metallothionein-II gene and a related processed gene.人类金属硫蛋白基因——金属硫蛋白-II基因及一个相关加工基因的一级结构
Nature. 1982 Oct 28;299(5886):797-802. doi: 10.1038/299797a0.
2
Germline integration of moloney murine leukemia virus at the Mov13 locus leads to recessive lethal mutation and early embryonic death.莫洛尼鼠白血病病毒在Mov13位点的种系整合导致隐性致死突变和早期胚胎死亡。
Cell. 1983 Jan;32(1):209-16. doi: 10.1016/0092-8674(83)90511-1.
3
Expression of a retrovirus encoding human HPRT in mice.
用于稳定生产自失活γ逆转录病毒的简单病毒/最小化猪尾巴病毒杂交载体。
BMC Res Notes. 2015 Aug 27;8:379. doi: 10.1186/s13104-015-1354-y.
4
The U3 region of Moloney murine leukemia virus contains position-independent cis-acting sequences involved in the nuclear export of full-length viral transcripts.莫洛尼鼠白血病病毒的U3区域包含参与全长病毒转录本核输出的位置独立顺式作用序列。
J Biol Chem. 2014 Jul 18;289(29):20158-69. doi: 10.1074/jbc.M113.545855. Epub 2014 May 30.
5
Nel positively regulates the genesis of retinal ganglion cells by promoting their differentiation and survival during development.Nel通过在发育过程中促进视网膜神经节细胞的分化和存活，正向调节其生成。
Mol Biol Cell. 2014 Jan;25(2):234-44. doi: 10.1091/mbc.E13-08-0453. Epub 2013 Nov 20.
6
Lentiviral and Moloney retroviral expression of green fluorescent protein in somatotrophs in vivo.体内生长激素细胞中绿色荧光蛋白的慢病毒和 Moloney 逆转录病毒表达。
PLoS One. 2013;8(1):e54437. doi: 10.1371/journal.pone.0054437. Epub 2013 Jan 16.
7
Effects of an internal transcription unit and its orientation on retrovirus titre and expression.内部转录单元及其方向对逆转录病毒效价和表达的影响。
Cytotechnology. 1997 May;24(1):31-8. doi: 10.1023/A:1007956013403.
8
A tunable dual-promoter integrator for targeting of cancer cells.一种用于靶向癌细胞的可调双启动子整合子。
Mol Syst Biol. 2010 Dec 21;6:444. doi: 10.1038/msb.2010.99.
9
Sustained inhibition of PKCα reduces intravasation and lung seeding during mammary tumor metastasis in an in vivo mouse model.持续抑制蛋白激酶 Cα可减少体内小鼠乳腺癌转移模型中的血管内渗和肺转移。
Oncogene. 2011 Jan 20;30(3):323-33. doi: 10.1038/onc.2010.415. Epub 2010 Sep 20.
10
Enhanced gene expression from retroviral vectors.逆转录病毒载体增强的基因表达。
BMC Biotechnol. 2008 Feb 25;8:19. doi: 10.1186/1472-6750-8-19.
编码人次黄嘌呤磷酸核糖转移酶的逆转录病毒在小鼠中的表达。
Science. 1984 Aug 10;225(4662):630-2. doi: 10.1126/science.6377498.
4
Transcriptional interference in avian retroviruses--implications for the promoter insertion model of leukaemogenesis.禽逆转录病毒中的转录干扰——对白血病发生的启动子插入模型的启示
Nature. 1984;307(5948):241-5. doi: 10.1038/307241a0.
5
Construction and applications of a highly transmissible murine retrovirus shuttle vector.一种高传染性小鼠逆转录病毒穿梭载体的构建与应用
Cell. 1984 Jul;37(3):1053-62. doi: 10.1016/0092-8674(84)90440-9.
6
Expression of complete chicken thymidine kinase gene inserted in a retrovirus vector.插入逆转录病毒载体中的完整鸡胸苷激酶基因的表达。
Mol Cell Biol. 1984 Apr;4(4):749-54. doi: 10.1128/mcb.4.4.749-754.1984.
7
Retrovirus transduction: generation of infectious retroviruses expressing dominant and selectable genes is associated with in vivo recombination and deletion events.逆转录病毒转导：表达显性和可选择基因的感染性逆转录病毒的产生与体内重组和缺失事件相关。
Mol Cell Biol. 1983 Dec;3(12):2180-90. doi: 10.1128/mcb.3.12.2180-2190.1983.
8
A transmissible retrovirus expressing human hypoxanthine phosphoribosyltransferase (HPRT): gene transfer into cells obtained from humans deficient in HPRT.一种表达人次黄嘌呤磷酸核糖转移酶（HPRT）的可传播逆转录病毒：基因转移至来自缺乏HPRT的人类细胞中。
Proc Natl Acad Sci U S A. 1983 Aug;80(15):4709-13. doi: 10.1073/pnas.80.15.4709.
9
Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase.人次黄嘌呤磷酸核糖基转移酶全长可表达cDNA的分离与鉴定
Proc Natl Acad Sci U S A. 1983 Jan;80(2):477-81. doi: 10.1073/pnas.80.2.477.
10
The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.劳氏肉瘤病毒长末端重复序列通过DNA介导转染导入多种真核细胞时是一个强启动子。
Proc Natl Acad Sci U S A. 1982 Nov;79(22):6777-81. doi: 10.1073/pnas.79.22.6777.