Department of Cardio- Respiratory Disease Biology, CSIR Institute of Genomics and Integrative Biology, Mathura Road, New Delhi-110025, India.
Department of Biological Sciences, Academy of Scientific and Innovative Research, CSIR- HRDC campus, Sector 19, Kamla Nehru Nagar, Ghaziabad- 201002, India.
RNA Biol. 2021 Nov 12;18(sup2):604-611. doi: 10.1080/15476286.2021.1989217. Epub 2021 Nov 8.
By analysis of lncRNA expression profiles of macrophages in response to (Mtb) infection, we identified novel highly expressed transcripts, unique in encompassing a protein coding transcript- Cytidine Monophosphate Kinase 2 (CMPK2) and a previously identified lncRNA- Negative Regulator of Interferon Response (NRIR). While these transcripts (TILT1, 2,3 - LR4 and nfection induced ong ranscript) are induced by virulent Mtb as well as lipopolysaccharide (LPS) early, lack of/delayed expression in non-viable Mtb/BCG infected cells, respectively, suggest an important role in macrophage responses. The elevated expression by 3 hr in response to fast growing bacteria further emphasizes the importance of these RNAs in the macrophage infection response. Overall, we provide evidence for the presence of multiple transcripts that form a part of the early infection response programme of macrophages. IFN: Interferon; NRIR: negative regulator of interferon response; CMPK2: cytidine/ uridine monophosphate kinase; LPS: lipopolysaccharide; LAM: Lipoarabinomannan; PIMs: Phosphatidylinositol Mannosides; TILT1, 2,3: LR4 and nfection induced ong ranscript; TLR4: Toll-like receptor 4; Mtb: ; BCG: BCG; MDMs: human monocyte derived macrophages.
通过分析巨噬细胞对（Mtb）感染的长链非编码 RNA 表达谱，我们鉴定出了新型高表达的转录本，这些转录本独特之处在于包含一个编码蛋白的转录本——胞苷一磷酸激酶 2（CMPK2）和一个先前鉴定的长链非编码 RNA——干扰素反应负调控因子（NRIR）。虽然这些转录本（TILT1、2、3-LR4 和感染诱导的长转录本）被毒力 Mtb 以及脂多糖（LPS）早期诱导，但在非存活 Mtb/BCG 感染细胞中分别缺乏/延迟表达，提示它们在巨噬细胞反应中具有重要作用。这些 RNA 在快速生长的细菌刺激下 3 小时即可显著上调，进一步强调了它们在巨噬细胞感染反应中的重要性。综上所述，我们提供了证据证明存在多个转录本，这些转录本构成了巨噬细胞早期感染反应程序的一部分。IFN：干扰素；NRIR：干扰素反应负调控因子；CMPK2：胞苷/尿苷一磷酸激酶；LPS：脂多糖；LAM：阿拉伯甘露聚糖脂；PIMs：磷酸化酰基肌醇甘露糖苷；TILT1、2、3-LR4 和感染诱导的长转录本；TLR4：Toll 样受体 4；Mtb：结核分枝杆菌；BCG：卡介苗；MDMs：人单核细胞衍生的巨噬细胞。
