Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, China.
Anim Biotechnol. 2023 Jun;34(3):619-632. doi: 10.1080/10495398.2021.1990940. Epub 2021 Nov 8.
The aim of this study was to identify genes related to precocious puberty expressed in the pituitary of goats at different growth stages by suppression subtractive hybridization (SSH). The pituitary glands from Jining Gray (JG) goats (early puberty) and Liaoning Cashmere (LC) goats (late puberty) at 30, 90, and 180days were used in this study. To identify differentially expressed genes (DEGs) in the pituitary glands, mRNA was extracted from these tissues, and SSH libraries were constructed and divided into the following groups: juvenile group (30-JG 30-LC, API), puberty group (90-JG 180-LC, BPI), and control group (90-JG 90-LC, EPI). A total of 60, 49, and 58 DEGs were annotated by 222 Gene Ontology (GO) terms and 75 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Most of the DEGs were significantly enriched in GO terms related to 'structural constituent of ribosome', 'translation' and 'GTP binding', and numerous DEGs were also significantly enriched in KEGG terms related to the Jak-STAT signaling and oocyte meiosis pathways. Candidate genes associated with precocious puberty and sexual development were screened from the SSH libraries. These genes were analyzed to determine if they were expressed in the pituitary tissues of the goats at different growth stages and to identify genes that may influence the hypothalamic-pituitary-gonadal (HPG) axis. In this study, we found precocious puberty-related genes (such as , , and ) that may be interesting from an evolutionary perspective and that could be investigated for use in future goat breeding programs. Our results provide a valuable dataset that will facilitate further research into the reproductive biology of goats.
本研究旨在通过抑制差减杂交（SSH）技术，鉴定不同生长阶段山羊垂体中表达的与性早熟相关的基因。该研究使用了济宁青山羊（JG）和辽宁绒山羊（LC）在 30、90 和 180 日龄的垂体组织。为了鉴定垂体组织中差异表达的基因（DEGs），从这些组织中提取 mRNA，并构建 SSH 文库，分为以下几组：幼年期组（30-JG 30-LC，API）、青春期组（90-JG 180-LC，BPI）和对照组（90-JG 90-LC，EPI）。通过 222 个基因本体论（GO）术语和 75 个京都基因与基因组百科全书（KEGG）途径注释了 60、49 和 58 个 DEGs。大多数 DEGs 显著富集于与“核糖体结构成分”、“翻译”和“GTP 结合”相关的 GO 术语中，许多 DEGs 也显著富集于与 Jak-STAT 信号和卵母细胞减数分裂途径相关的 KEGG 术语中。从 SSH 文库中筛选与性早熟和性发育相关的候选基因。对这些基因进行分析，以确定它们是否在不同生长阶段的山羊垂体组织中表达，并鉴定可能影响下丘脑-垂体-性腺（HPG）轴的基因。在本研究中，我们发现了一些与性早熟相关的基因（如 、 和 ），这些基因可能具有进化意义，值得进一步研究，也可用于未来的山羊育种计划。我们的研究结果提供了一个有价值的数据集，将有助于进一步研究山羊的生殖生物学。
