Cao G L, Feng T, Chu M X, Di R, Zhang Y L, Huang D W, Liu Q Y, Hu W P, Wang X Y
Reprod Fertil Dev. 2015 May 15. doi: 10.1071/RD14434.
The cost of developing replacement nanny goats could be reduced by decreasing the age at puberty because this way nanny goats could be brought into production at an earlier age. The aim of the present study was to screen genes related to puberty to investigate the molecular mechanisms of puberty. Subtracted cDNA libraries were constructed for hypothalami from juvenile (Group A), pubertal (Group B) and age-matched control pubertal (Group E) Jining grey (JG) and Liaoning cashmere (LC) goats using suppression subtractive hybridisation (SSH). Differentially expressed genes were analysed by bioinformatics methods. There were 203 expressed sequence tags (ESTs) in the subtracted cDNA libraries that were differentially expressed between JG and LC goats at the juvenile stage, 226 that were differentially expressed at puberty and 183 that were differentially expressed in the age-matched control group. The differentially expressed ESTs in each subtracted cDNA library were classified as known gene, known EST and unknown EST according to sequence homology in the GenBank non-redundant (NR) and EST database. According to gene function analysis in the COG (Cluster of Orthologous Groups) database, the known genes were grouped into 10 subdivisions in Group A, into seven subdivisions in Group E and into nine subdivisions in Group B under three categories: cellular processes and signalling, information storage and processing, and metabolism. Pathway analysis in the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway database of known genes revealed that the three pathways that most differentially expressed genes were involved in were metabolic pathways, Parkinson's disease and oxidative phosphorylation. Protein interaction analysis of the high homology genes revealed the most dominant network to be structure of ribosome/protein translation, oxidative phosphorylation and carbohydrate metabolism. The results reveal that the onset of puberty is a complex event involving multiple genes in multiple biological processes. The differentially expressed genes include genes related to both neuroendocrine and energy metabolism.
通过降低初情期年龄可降低培育后备母羊的成本，因为这样母羊能更早进入生产阶段。本研究的目的是筛选与初情期相关的基因，以探究初情期的分子机制。利用抑制性消减杂交（SSH）技术，构建了幼年（A组）、青春期（B组）以及年龄匹配的青春期对照（E组）济宁青山羊（JG）和辽宁绒山羊（LC）下丘脑的消减cDNA文库。采用生物信息学方法分析差异表达基因。在消减cDNA文库中，幼年阶段JG和LC山羊之间有203个表达序列标签（EST）差异表达，青春期有226个差异表达，年龄匹配对照组中有183个差异表达。根据GenBank非冗余（NR）和EST数据库中的序列同源性，将每个消减cDNA文库中的差异表达EST分为已知基因、已知EST和未知EST。根据直系同源基因簇（COG）数据库中的基因功能分析，已知基因在A组中分为10个亚类，E组中分为7个亚类，B组中分为9个亚类，共三大类：细胞过程与信号传导、信息存储与处理以及代谢。对已知基因在京都基因与基因组百科全书（KEGG）通路数据库中的通路分析表明，差异表达基因涉及最多的三条通路是代谢通路、帕金森病和氧化磷酸化。对高同源性基因的蛋白质相互作用分析表明，最主要的网络是核糖体/蛋白质翻译结构、氧化磷酸化和碳水化合物代谢。结果表明，初情期的开始是一个复杂的事件，涉及多个生物学过程中的多个基因。差异表达基因包括与神经内分泌和能量代谢相关的基因。
