Laboratório de Patologia de Moléstias Infecciosas (LIM-50), Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.
Instituto de Investigaciones en Microbiología, Universidad Nacional Autónoma de Honduras, Tegucigalpa, Honduras.
Parasite Immunol. 2022 Jan;44(1-2):e12896. doi: 10.1111/pim.12896. Epub 2021 Nov 17.
In some central-American countries, Leishmania (L.) infantum chagasi infection can cause non-ulcerated or atypical cutaneous leishmaniasis (NUCL) in addition to the classic clinical form, visceral leishmaniasis (VL). Little is known about the host-parasite relationship that can contribute to the determination of one or another clinical form. The present study had the objective to evaluate the humoral and cellular immunity in the sera of individuals affected by NUCL to improve the comprehension of this atypical host-parasite interaction. Based on clinical and laboratory diagnosis, serum of 80 individuals was collected to evaluate the cytokines and immunoglobulins profile of NUCL (n = 47), VL patients (n = 5), and negative controls (n = 28). Cytokines were detected using Cytokine Bead Array (CBA) Human Th1/Th2/Th17 kit according to the manufacturer's instructions; class (IgG and IgM), and subclass of (IgG1 and IgG2) immunoglobulins was evaluated by ELISA using specific antigens. The concentration of TNF-α, IFN-γ, IL-2 and IL-4 cytokines in NUCL, VL and control was present below the detection threshold of CBA kit. IL-6, IL-10 and IL-17A cytokines was lower in NUCL compared to LV patients. Regarding to immunoglobulins, NUCL patients produced 4.0 times more IgG than the control, while VL patients produced 6.6 times more; and IgM level was 1.6 times higher in NUCL and 2.6 times in VL patients compared to the control. Concerning the immunoglobulins subclass, only VL patients showed positive reaction for IgG1, and IgG2 did not show positive reaction among the groups. The results showed a weak cellular and humoral systemic immune response in NUCL patients.
在一些中美洲国家，感染利什曼原虫（L.）婴儿查加斯亚种除了经典的内脏利什曼病（VL）临床形式外，还可导致非溃疡性或非典型皮肤利什曼病（NUCL）。人们对宿主 - 寄生虫关系知之甚少，这种关系有助于确定一种或另一种临床形式。本研究的目的是评估受 NUCL 影响的个体血清中的体液和细胞免疫，以提高对这种非典型宿主 - 寄生虫相互作用的理解。根据临床和实验室诊断，收集了 80 个人的血清，以评估 NUCL（n=47）、VL 患者（n=5）和阴性对照（n=28）的细胞因子和免疫球蛋白谱。根据制造商的说明，使用 Cytokine Bead Array（CBA）人 Th1/Th2/Th17 试剂盒检测细胞因子；通过 ELISA 使用特定抗原评估类（IgG 和 IgM）和亚类（IgG1 和 IgG2）免疫球蛋白。在 NUCL、VL 和对照中，TNF-α、IFN-γ、IL-2 和 IL-4 细胞因子的浓度低于 CBA 试剂盒的检测阈值。与 LV 患者相比，NUCL 患者的 IL-6、IL-10 和 IL-17A 细胞因子水平较低。关于免疫球蛋白，NUCL 患者产生的 IgG 是对照组的 4.0 倍，而 VL 患者产生的 IgG 是对照组的 6.6 倍；与对照组相比，NUCL 和 VL 患者的 IgM 水平分别高 1.6 倍和 2.6 倍。关于免疫球蛋白亚类，只有 VL 患者对 IgG1 呈阳性反应，而在各组中均未观察到 IgG2 的阳性反应。结果表明，NUCL 患者的细胞和体液全身免疫反应较弱。
