Laboratory of Biomass & Bioenergy, State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, Hubei University, Wuhan, Hubei Province, China.
Laboratory of Biomass & Bioenergy, State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, Hubei University, Wuhan, Hubei Province, China; National-Local Joint Engineering Research Center of Biomass Refining and High-Quality Utilization, Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, Advanced Catalysis and Green Manufacturing Collaborative Innovation Center, School of Pharmacy, Changzhou University, Changzhou, Jiangsu Province, China.
Bioresour Technol. 2022 Jan;344(Pt B):126299. doi: 10.1016/j.biortech.2021.126299. Epub 2021 Nov 6.
2,5-Bis(hydroxymethyl)furan (BHMF) is one kind of important upgraded derivatives of biobased 5-hydroxymethylfuran (5-HMF). This study verified the feasibility of one-pot chemoenzymatic conversion of biobased D-fructose to BHMF by cascade catalysis with deep eutectic solvent Lactic acid:Betaine (LA:B) and reductase biocatalyst in LA:B - HO. Using D-fructose (36.0 g/L) as feedstock, the yield of 5-HMF reached 91.6% in DES LA:B - HO (15:85, v:v) at 150 °C for 1.5 h. Using D-fructose (2 mol D-fructose/mol 5-HMF) as cosubstrate, commercial 5-HMF (125 mM) was converted into BHMF at 90.7% yield by whole-cells of Pseudomonas putida S12 within 24 h at 30 °C and pH 8.0. In addition, Pseudomonas Putida S12 could efficiently transform D-fructose-valorized 5-HMF into BHMF [98.4% yield, based on 5-HMF; 90.1% yield, based on substrate D-fructose] in DES LA:B - HO. An efficient chemoenzymatic valorization of D-fructose to BHMF was developed in a benign reaction system.
2,5-双羟甲基呋喃（BHMF）是一种重要的生物基 5-羟甲基糠醛（5-HMF）衍生升级产物。本研究通过使用深共熔溶剂乳酸：甜菜碱（LA：B）和还原酶生物催化剂在 LA：B-HO 中进行级联催化，验证了一锅法化学酶法将生物基 D-果糖转化为 BHMF 的可行性。以 D-果糖（36.0 g/L）为原料，在 150°C 下反应 1.5 h，DES LA：B-HO（15:85，v：v）中 5-HMF 的产率达到 91.6%。以 2 mol D-果糖/mol 5-HMF 为共底物，在 30°C 和 pH 8.0 下，利用恶臭假单胞菌 S12 的全细胞在 24 h 内将商业 5-HMF（125 mM）转化为 BHMF，产率为 90.7%。此外，恶臭假单胞菌 S12 可以在 DES LA：B-HO 中有效地将 D-果糖增值的 5-HMF 转化为 BHMF [基于 5-HMF 的 98.4%产率；基于底物 D-果糖的 90.1%产率]。在良性反应体系中开发了一种从 D-果糖高效制备 BHMF 的化学酶法。
