大肠杆菌 CCZU-K14 全细胞生物合成生物质衍生的 5-羟甲基糠醛为 2,5-双(羟甲基)呋喃。
Biological synthesis of 2,5-bis(hydroxymethyl)furan from biomass-derived 5-hydroxymethylfurfural by E. coli CCZU-K14 whole cells.
机构信息
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, Changzhou University, Changzhou, China; Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, China.
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, Changzhou University, Changzhou, China.
出版信息
Bioresour Technol. 2018 Jan;247:1215-1220. doi: 10.1016/j.biortech.2017.09.071. Epub 2017 Sep 12.
Biocatalytic upgrading of bio-based platform chemical 5-hydroxymethylfurfural (5-HMF) to 2,5-bis(hydroxymethyl)furan (BHMF) is currently of great interest due to the product specificity, mild reaction and high efficiency. In this work, 200mM 5-HMF could be effectively biotransformed to BHMF at 90.6% with highly 5-HMF-tolerant recombinant E. coli CCZU-K14 whole cells at pH 6.5 and 30°C under the optimum reaction conditions (cosubstrate glucose 1.0mol glucose/(mol 5-HMF), D-xylose 400mM, l-glutamic acid 250mM, Mg 1.5mM, 0.2mol β-cyclodextrin/(mol 5-HMF), CTAB (cetyltrimethyl ammonium bromide) 12.5mM, and 0.1g wet cells/mL). It was found that E. coli CCZU-K14 was highly tolerant to 5-HMF (up to 400mM). Effective bioreduction of biomass-derived 5-HMF (≤200) to BHMF was successfully demonstrated in this study. In conclusion, this strategy showed high potential application for the synthesis of BHMF.
生物催化将生物基平台化学品 5-羟甲基糠醛（5-HMF）升级为 2,5-双羟甲基呋喃（BHMF）目前引起了极大的关注，因为该反应具有产物特异性、温和的反应条件和高效率。在这项工作中，在最佳反应条件下（辅助底物葡萄糖 1.0mol 葡萄糖/（mol 5-HMF），D-木糖 400mM，l-谷氨酸 250mM，Mg 1.5mM，0.2mol β-环糊精/（mol 5-HMF），十六烷基三甲基溴化铵（CTAB）12.5mM，和 0.1g 湿细胞/mL），pH 值为 6.5，温度为 30°C，高耐 5-HMF 的重组大肠杆菌 CCZU-K14 全细胞可以有效地将 200mM 的 5-HMF 转化为 BHMF，转化率为 90.6%。研究发现大肠杆菌 CCZU-K14 对 5-HMF（高达 400mM）具有很高的耐受性。本研究成功地证明了生物质衍生的 5-HMF（≤200）有效生物还原为 BHMF。总之，该策略显示出了在 BHMF 合成方面的高应用潜力。
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