Zhang Shunli, Wu Changqing, Ma Cuiluan, Li Lei, He Yu-Cai
School of Pharmacy, Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, National-Local Joint Engineering Research Center of Biomass Refining and High-Quality Utilization, Changzhou University, Changzhou, China; State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, China.
State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, China.
Bioresour Technol. 2023 Mar;371:128579. doi: 10.1016/j.biortech.2023.128579. Epub 2023 Jan 4.
Via combination catalysis with deep eutectic solvent lactic acid:betaine (chemocatalyst) and HMFOMUT cell (biocatalyst: E. coli HMFOMUT whole-cell), one-pot manufacture of 2,5-furandimethanol from waste bioresource was constructed in a chemoenzymatic approach. With bread waste (50 g/L) as substrate, the 5-hydroxymethylfuran yield reached 44.2 Cmol% (based on bread waste) by lactic acid:betaine (15 wt%) at 180 °C for 15 min. With glucose as co-substrate, HMFOMUT could transform 5-hydroxymethylfurfural (150 mM) to 2,5-furandimethanol (84.5 % yield) after 1 day at 37 °C and pH 7.0. In lactic acid:betaine-HO, HMFOMUT effectively converted bread-derived 5-hydroxymethylfurfural into 2,5-furandimethanol in a productivity of 700 kg 2,5-furandimethanol per kg 5-hydroxymethylfurfural (230 kg 2,5-furandimethanol per kg bread). In an eco-friendly lactic acid:betaine system, an effective one-pot chemoenzymatic strategy was firstly developed to convert bread waste into 2,5-furandimethanol, which would reduce the operation cost and has potential application value for valorizing waste food bioresource into value-added furan.
甜菜碱（化学催化剂）和HMFOMUT细胞（生物催化剂：大肠杆菌HMFOMUT全细胞）的组合催化，采用化学酶法构建了从废弃生物资源一锅法制备2,5-呋喃二甲醇的工艺。以面包废料（50 g/L）为底物，在180℃下用乳酸：甜菜碱（15 wt%）反应15分钟，5-羟甲基糠醛产率达到44.2 Cmol%（基于面包废料）。以葡萄糖为共底物，在37℃和pH 7.0条件下反应1天后，HMFOMUT可将5-羟甲基糠醛（150 mM）转化为2,5-呋喃二甲醇（产率84.5%）。在乳酸：甜菜碱-HO体系中，HMFOMUT能以每千克5-羟甲基糠醛700千克2,5-呋喃二甲醇（每千克面包230千克2,5-呋喃二甲醇）的生产率，将面包来源的5-羟甲基糠醛有效转化为2,5-呋喃二甲醇。在环保的乳酸：甜菜碱体系中，首次开发了一种有效的一锅法化学酶策略，将面包废料转化为2,5-呋喃二甲醇，这将降低运营成本，对于将废弃食品生物资源转化为增值呋喃具有潜在的应用价值。
