CAS and Shandong Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, PR China.
CAS and Shandong Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
Bioresour Technol. 2022 Jan;344(Pt B):126275. doi: 10.1016/j.biortech.2021.126275. Epub 2021 Nov 6.
In the present study, the mechanisms for facilitating fatty acid and astaxanthin biosynthesis-related processes by inhibiting the alternative oxidase (AOX) respiratory pathway in Haematococcus pluvialis was investigated. The restriction of the AOX pathway induced the accumulation of reactive oxygen species, NAD(P)H and its substrates (acetyl-CoA, pyruvate and glyceraldehy-3-phosphate), which are required for fatty acid and astaxanthin production, thereby promoting the carbon flux into fatty acid and astaxanthin biosynthesis. During a 9-day incubation period, the fatty acid and astaxanthin contents increased by 20.6% and 20.7%, respectively, when the AOX pathway was inhibited approximately 37.7%. The AOX pathway may be inhibited by nutrient (nitrogen and phosphorus) removal, inhibitor addition and air/CO aeration adjustments in the large-scale cultivation of H. pluvialis. Therefore, the current study provides a useful enhancement strategy for fatty acid and astaxanthin coproduction and elucidates the roles of the AOX pathway in regulating fatty acid and astaxanthin biosynthesis.
在本研究中，通过抑制雨生红球藻中的交替氧化酶（AOX）呼吸途径，研究了促进脂肪酸和虾青素生物合成相关过程的机制。AOX 途径的限制诱导了活性氧、NAD(P)H 及其底物（乙酰辅酶 A、丙酮酸和甘油醛-3-磷酸）的积累，这些物质是脂肪酸和虾青素生产所必需的，从而促进了碳通量进入脂肪酸和虾青素生物合成。在 9 天的孵育期内，当 AOX 途径被抑制约 37.7%时，脂肪酸和虾青素的含量分别增加了 20.6%和 20.7%。AOX 途径可能会被雨生红球藻大规模培养中的营养（氮和磷）去除、抑制剂添加和空气/CO 曝气调整所抑制。因此，本研究为脂肪酸和虾青素的共生产提供了一种有用的增强策略，并阐明了 AOX 途径在调节脂肪酸和虾青素生物合成中的作用。
