Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.
Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.
Res Vet Sci. 2021 Dec;141:146-155. doi: 10.1016/j.rvsc.2021.10.026. Epub 2021 Nov 2.
Porcine epidemic diarrhea virus (PEDV) is a deadly pathogen that still plagues suckling piglets. However, there is still no anti-PEDV drug available in clinics. To develop potential anti-PEDV drugs, the antiviral activity of Alpiniae oxyphyllae fructus polysaccharide 3 (AOFP3) against PEDV infection in IPEC-J2 cells were assessed in our present study. The structural characterization of AOFP3 was studied by using HPAEC, GC-MS, FT-IR and NMR techniques. At the same time, the anti-PEDV activity of AOFP3 was investigated by performing RT-qPCR, Western blot and immunofluorescence assays. The results showed that AOFP3 (44.4 kDa) was composed of glucose and galacturonic acid at a molar ratio of 77.54:22.46 and consisted of →4)-α-D-Glcp-(1→, →4,6)-α-D-Glcp-(1→, T-α-D-Glcp-(1→ and →4)-α-D-GalAp-(1→. AOFP3 significantly decreased PEDV titer in IPEC-J2 cells and prevented cellular damage of IPEC-J2 cells caused by PEDV infection. Furthermore, AOFP3 showed an antioxidative activity in inhibiting PEDV reproduction. Therefore, AOFP3 was expected to be a material of anti-PEDV drug.
猪流行性腹泻病毒（PEDV）是一种致命的病原体，仍然困扰着哺乳期仔猪。然而，临床上仍然没有抗 PEDV 的药物。为了开发潜在的抗 PEDV 药物，本研究评估了益智仁多糖 3（AOFP3）对 IPEC-J2 细胞中 PEDV 感染的抗病毒活性。通过 HPAEC、GC-MS、FT-IR 和 NMR 技术研究了 AOFP3 的结构特征。同时，通过 RT-qPCR、Western blot 和免疫荧光分析研究了 AOFP3 的抗 PEDV 活性。结果表明，AOFP3（44.4 kDa）由葡萄糖和半乳糖醛酸组成，摩尔比为 77.54:22.46，由 →4)-α-D-Glcp-(1→、→4,6)-α-D-Glcp-(1→、T-α-D-Glcp-(1→和 →4)-α-D-GalAp-(1→组成。AOFP3 显著降低了 IPEC-J2 细胞中的 PEDV 滴度，并防止了 PEDV 感染引起的 IPEC-J2 细胞损伤。此外，AOFP3 在抑制 PEDV 复制方面表现出抗氧化活性。因此，AOFP3 有望成为抗 PEDV 药物的一种物质。
