Li Lan, Fu Meiling, Yang Deyuan, Tu Yifeng, Yan Jilin
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Industrial Park, Suzhou 215123, China.
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Industrial Park, Suzhou 215123, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Feb 15;267(Pt 1):120563. doi: 10.1016/j.saa.2021.120563. Epub 2021 Oct 29.
A method for a sensitive fluorescence detection of glutathione was established. Glutathione-stabilized copper nanoclusters (CuNCs) were synthesized via a facile process. These CuNCs showed blue fluorescence with a peak around 450 nm. In the presence of p-benzoquinone (PBQ), the electron transfer from the copper nanoclusters to PBQ quenched the fluorescence of the CuNCs. Glutathione (GSH), as a reducing agent, formed a complex with PBQ. This formation inhibited the quenching from PBQ, and a restored fluorescence was obtained. This interaction provided a fluorescence enhancement for the measurement of GSH. Under the optimal condition, linear responses were obtained toward GSH in the ranges of 0.06-6.0 μM, with a limit of detection at 20 nM. This developed assay was easy in operation with high sensitivity and selectivity. The applicability was approved with successful glutathione measurements in real samples.
建立了一种灵敏检测谷胱甘肽的荧光方法。通过简便的过程合成了谷胱甘肽稳定的铜纳米簇（CuNCs）。这些CuNCs发出蓝色荧光，峰值约为450nm。在对苯醌（PBQ）存在下，从铜纳米簇到PBQ的电子转移淬灭了CuNCs的荧光。谷胱甘肽（GSH）作为还原剂，与PBQ形成复合物。这种形成抑制了PBQ的淬灭作用，从而获得了恢复的荧光。这种相互作用为GSH的测量提供了荧光增强。在最佳条件下，对GSH在0.06 - 6.0μM范围内获得线性响应，检测限为20 nM。所开发的检测方法操作简便，具有高灵敏度和选择性。通过在实际样品中成功测量谷胱甘肽，验证了其适用性。
