Division of Physical Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand; Center of Excellence for Trace Analysis and Biosensor, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
Division of Physical Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand; Center of Excellence for Trace Analysis and Biosensor, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand; Thailand Center of Excellence in Physics, Commission on Higher Education, 328 Si Ayutthaya Road, Bangkok 10400, Thailand.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Apr 5;270:120816. doi: 10.1016/j.saa.2021.120816. Epub 2021 Dec 29.
A fluorescent probe based on glutathione-capped copper nanoclusters (GSH-CuNCs) was developed for the detection of dual targets, human serum albumin (HSA) and creatinine, in human urine. The GSH-CuNCs were synthesized by a one-pot green method using ascorbic acid as a reducing agent. The detection of HSA was in a turn-on mode via electrostatic interaction in a basic condition while the detection of creatinine was in a turn-off mode via non-covalent bonding in an acidic condition. Under optimal conditions, the linear range and detection limit of HSA were 5.0 nM to 150 nM and 1.510 ± 0.041 nM, while those of creatinine were 30 μM to 1000 μM and 13.0 ± 1.0 μM. This easily fabricated nanocluster probe provided a fast response with high sensitivity, and good selectivity. Recoveries from urine samples were in the range of 81.44 ± 0.25 to 109.22 ± 0.57% for HSA and 80.57 ± 0.16 to 109.0 ± 0.10% for creatinine. The urinary analytical results from the fluorescent probe were in good agreement (P > 0.05) to those obtained from immunoturbidimetric and enzymatic methods, signifying the excellent performance of this sensing system.
一种基于谷胱甘肽包裹的铜纳米簇（GSH-CuNCs）的荧光探针被开发用于检测人尿液中的双重靶标，即人血清白蛋白（HSA）和肌酸酐。GSH-CuNCs 通过使用抗坏血酸作为还原剂的一锅法绿色方法合成。在碱性条件下通过静电相互作用以开启模式检测 HSA，而在酸性条件下通过非共价键合以关闭模式检测肌酸酐。在最佳条件下，HSA 的线性范围和检测限分别为 5.0 nM 至 150 nM 和 1.510 ± 0.041 nM，而肌酸酐的线性范围和检测限分别为 30 μM 至 1000 μM 和 13.0 ± 1.0 μM。这种易于制备的纳米簇探针具有高灵敏度和良好选择性的快速响应。HSA 的尿液回收率范围为 81.44 ± 0.25%至 109.22 ± 0.57%，肌酸酐的回收率范围为 80.57 ± 0.16%至 109.0 ± 0.10%。荧光探针的尿分析结果与免疫比浊法和酶法的结果非常吻合（P＞0.05），表明该传感系统具有优异的性能。
