Facultad de Química, Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria, 04510, Mexico City, México.
Centro Tecnológico, Facultad de Estudios Superiores (FES-Aragón), State of Mexico, Universidad Nacional Autónoma de México (UNAM), 57130, Aragon, México.
J Fluoresc. 2023 Sep;33(5):2041-2059. doi: 10.1007/s10895-023-03201-7. Epub 2023 Mar 28.
The present work highlights the sustainable approach for the transformation of plastic waste into fluorescent carbon dots (CDs) through carbonization and then they were functionalized with L-cysteine and o-phenylenediamine. CDs which were characterized by different analytical techniques such as X-ray diffraction (XRD), thermogravimetric analysis (TGA), Scanning electron microscopy (SEM), and transmission electron microscopy (TEM) are employed to recognize Cu, Fe, and Hg ions. The results show that the fluorescence emission was considerably quenched, and it is consistent with the interference and Jobs plots. The detection limit was found to be 0.35µM for Cu(II), 1.38 µM for Hg(II), and 0.51µM Fe(III). The interaction of CDs with metal ions enhances the fluorescence intensity detecting histamine successfully. It shows that plastic waste-based CDs can be applied clinically to detect toxic metals and biomolecules. Moreover, the system was employed to develop the cellular images using Saccharomyces cerevisiae cells with the support of a confocal microscope. Furthermore, theoretical studies were performed for the naphthalene layer (AR) as a model for C-dots, then optimized its structure and analyzed by using the molecular orbital. The obtained TD-DFT spectra coincided with experimental spectra for CDs/M/histamine systems.
本工作通过碳化将塑料废物转化为荧光碳点（CDs），强调了可持续的方法，然后用 L-半胱氨酸和邻苯二胺对其进行功能化。通过 X 射线衍射（XRD）、热重分析（TGA）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）等不同分析技术对 CDs 进行了表征，用于识别 Cu、Fe 和 Hg 离子。结果表明，荧光发射明显猝灭，与干扰和作业图一致。检测限分别为 0.35µM 的 Cu(II)、1.38µM 的 Hg(II)和 0.51µM 的 Fe(III)。CDs 与金属离子的相互作用增强了荧光强度，成功检测了组胺。这表明基于塑料废物的 CDs 可用于临床检测有毒金属和生物分子。此外，该系统还利用共聚焦显微镜支持 Saccharomyces cerevisiae 细胞来开发细胞图像。此外，还对萘层（AR）作为 CDs 的模型进行了理论研究，然后对其结构进行了优化，并通过分子轨道进行了分析。获得的 TD-DFT 光谱与 CDs/M/histamine 系统的实验光谱吻合。
