Department of Gynecology, Etlik Zubeyde Hanim Women's Health Training and Research Hospital, Faculty of Medicine, University of Health Sciences, Ankara, Turkey.
Department of Pathology, Etlik Zubeyde Hanim Women's Health Training and Research Hospital, Faculty of Medicine, University of Health Sciences, Ankara, Turkey.
Gynecol Obstet Invest. 2021;86(6):479-485. doi: 10.1159/000519195. Epub 2021 Nov 8.
The aim of the study was to evaluate the negative effect of nonionizing radiation on the treatment of endometrial hyperplasia (EH) with oral progesterone.
Forty oophorectomized Wistar Albino female rats were included in this experimental rat study.
The 4 groups were planned as follows: Group A; sham group; Group B; group receiving oral estradiol hemihydrate 4 mg/kg/day; Group C; 4 mg/kg/day oral estradiol hemihydrate followed with 1 mg/day medroxy progesterone acetate (MPA) and Group D; 4 mg/kg/day oral estradiol hemihydrate followed with 1 mg/day MPA with exposure to nonionizing radiation at 1800 mHz/3 h/day. After the experimental model, uterine horns were sampled and the preparations were evaluated for pathological parameters (glandular density, epithelial cell length, and luminal epithelial cell length) via light microscopy. Nonionizing radiation was created by a signal generator and a compatible mobile phone.
Estrogen was found to increase all parameters related to EH (p < 0.05). Progesterone treatment was found to decrease parameters related to EH (Group B vs. C; luminal epithelial cell length, glandular density, and epithelial length; 11.2 vs. 13.2 μm p = 0.007; 32.5 vs. 35.5, p = 0.068; and 219.9 μm vs. 285 µm, p < 0.001, respectively). Final analyses revealed reduced effectiveness of progesterone treatment in the rats exposed to nonionizing radiation (Group C vs. D); luminal epithelial cell length, glandular density, and epithelial length (11.2 μm vs. 13.5 μm, p = 0.179; 32.5 vs. 52, p < 0.001; and 219.9 μm vs. 374.1 μm, p = 0.001, respectively).
The limitations of our study are that the results of animal experiments may not be appropriate for direct adaptation to humans and the relatively low number of rats included in the study.
Nonionizing radiation reduces the effect of progesterone in patients receiving treatment for EH.
本研究旨在评估非电离辐射对口服孕激素治疗子宫内膜增生（EH）的负面影响。
将 40 只去卵巢 Wistar 白化雌性大鼠纳入本实验性大鼠研究。
将 4 组计划如下：A 组：假手术组；B 组：每天口服 4mg/kg 雌二醇半水合物；C 组：每天口服 4mg/kg 雌二醇半水合物，随后每天 1mg 醋酸甲羟孕酮（MPA）；D 组：每天口服 4mg/kg 雌二醇半水合物，随后每天 1mg 醋酸甲羟孕酮，并在 1800MHz/3h/d 下接受非电离辐射。在实验模型后，取样子宫角，并通过光学显微镜评估病理参数（腺体密度、上皮细胞长度和腔上皮细胞长度）。非电离辐射由信号发生器和兼容的移动电话产生。
发现雌激素增加了与 EH 相关的所有参数（p<0.05）。孕激素治疗被发现降低了与 EH 相关的参数（B 组与 C 组；腔上皮细胞长度、腺体密度和上皮长度；11.2μm 与 13.2μm，p=0.007；32.5μm 与 35.5μm，p=0.068；219.9μm 与 285μm，p<0.001）。最终分析表明，在接受非电离辐射的大鼠中，孕激素治疗的效果降低（C 组与 D 组）；腔上皮细胞长度、腺体密度和上皮长度（11.2μm 与 13.5μm，p=0.179；32.5μm 与 52μm，p<0.001；219.9μm 与 374.1μm，p=0.001）。
本研究的局限性在于，动物实验的结果可能不适合直接应用于人类，以及研究中纳入的大鼠数量相对较少。
非电离辐射降低了接受 EH 治疗的患者中孕激素的疗效。
