Sivas Mustafa Can, Tapisiz Omer Lutfi, Ayik Rasit Tan, Kahraman Devrim, Kiykac Altinbas Sadiman, Moraloglu Tekin Ozlem
Department of Obstetrics and Gynecology, University of Health Sciences, Etlik Zubeyde Hanim Women's Health Training and Research Hospital, Ankara, Turkey.
Department of Pathology, School of Medicine, The Union of Chambers and Commodity Exchanges of Turkey, University of Economics and Technology (TOBB ETU), Ankara, Turkey.
Heliyon. 2020 Oct 3;6(10):e05142. doi: 10.1016/j.heliyon.2020.e05142. eCollection 2020 Oct.
Endometrial hyperplasia is a process of endometrial proliferation that results in a thickening of the endometrial tissue. Melatonin might be able to change the pathophysiological process and prognosis into a positive way that might prevent and heal endometrial hyperplasia, which is the first stage of endometrial cancer. For this perspective, we tried to investigate the effect of melatonin on uterine hypertrophy/hyperplasia in an experimental rat model. Forty Wistar-Albino rats were undergone bilateral oophorectomy and randomized into four groups. To create a model of uterine hypertrophy/hyperplasia in all groups, except the control group [C] (n = 10), 4 mg/kg/day estradiol hemihydrate were given for 14 days. The uterine hypertrophy/hyperplasia was evaluated histopathologically in the left uterine horns, then the groups were treated for 14 days as follows; melatonin (10 mg/kg/day/po) [M] (n = 10), melatonin + estradiol hemihydrate (10 mg/kg/day/po and 4 mg/kg/day/po) [M + E] (n = 10), and dark environment [D] (n = 10). Finally, the effects of the melatonin were examined histopathologically in the right uterine horns. An uterine hypertrophy/hyperplasia model was established in all groups compared to the control group (p < 0.05). In the [M] and [M + E] groups, epithelial cell height and luminal epithelial cell height significantly decreased (41μm vs 12μm, p = 0.005; 14μm vs 10μm, p = 0.005, respectively for [M] group) and (32μm vs 14μm, p = 0.012; 17μm vs 10μm, p = 0.017, respectively for [M + E] group). The [D] group exhibited a significant decrease in epithelial cell height (33μm vs 20μm, p = 0.017). With or without estrogen exposure, melatonin-treated and physiologically melatonin-released rats experienced a significant uterine hypertrophy/hyperplasia recovery. Melatonin may have protective effects on endometrial hyperplasia.
子宫内膜增生是子宫内膜增殖的一个过程，会导致子宫内膜组织增厚。褪黑素或许能够以一种积极的方式改变病理生理过程和预后，从而预防和治愈子宫内膜增生，而子宫内膜增生是子宫内膜癌的第一阶段。基于此观点，我们试图在实验大鼠模型中研究褪黑素对子宫肥大/增生的影响。40只Wistar - 白化大鼠接受双侧卵巢切除术，并随机分为四组。为在除对照组[C]（n = 10）之外的所有组中建立子宫肥大/增生模型，给予4mg/kg/天的半水合雌二醇，持续14天。对左子宫角进行组织病理学评估子宫肥大/增生情况，然后各小组按如下方式治疗14天：褪黑素（10mg/kg/天/口服）[M]（n = 10）、褪黑素 + 半水合雌二醇（10mg/kg/天/口服和4mg/kg/天/口服）[M + E]（n = 10）以及黑暗环境[D]（n = 10）。最后，在右子宫角进行组织病理学检查褪黑素的作用。与对照组相比，所有组均建立了子宫肥大/增生模型（p < 0.05）。在[M]组和[M + E]组中，上皮细胞高度和腔上皮细胞高度显著降低（[M]组分别为41μm对12μm，p = 0.005；14μm对10μm，p = 0.005）以及（[M + E]组分别为32μm对14μm，p = 0.012；17μm对10μm，p = 0.017）。[D]组上皮细胞高度显著降低（33μm对20μm，p = 0.017）。无论是否暴露于雌激素，经褪黑素处理以及生理释放褪黑素的大鼠子宫肥大/增生均有显著恢复。褪黑素可能对子宫内膜增生具有保护作用。
