Wang Qiguo, Mou Wenting, Luo Tao, Baokaixi Guliayi, Luo Yongjun, Xiaokaiti Maidina, Saimaiti Xiaowukaiti, Wang Xinhui, Hao Junhui, Gui Youjun, Wang Xiaojun, Wu Haiyan, Wang Xijiang
Department of Emergency Response and Plague Control, Xinjiang Center for Disease Control and Prevention, Urumqi, 830000, China.
Xinjiang Key Laboratory of Vector-borne Infectious Diseases, Urumqi, 830000, China.
Heliyon. 2024 Jun 19;10(12):e33005. doi: 10.1016/j.heliyon.2024.e33005. eCollection 2024 Jun 30.
In the Junggar Basin plague focus, and live together, and their parasitic fleas have a similar community structure. However, has significantly higher positive rates of and anti-F1 antibody compared with . In this study, - and antiF1 antibody-negative and were collected in Qitai county, Fukang city and Mulei county of the eastern part of the Junggar Basin. The genomic DNA was extracted from their spleen tissues, and RT1-Db1exon1 was amplified through PCR procedure and then sequenced. Sequence analysis was performed and molecular diversity parameters were calculated and compared. The results showed that there were significant differences in nucleotide composition, amino acid composition, number and distribution of single nucleotide polymorphism (SNP) sites and number of haplotypes between and . The nucleotide diversity () for was 0.00420 ± 0.00139, the haplotype diversity () was 0.833 ± 0.086, and the average number of nucleotide differences () was 2.02564. The for was 0.06569 ± 0.02524, and the was 1.000 ± 0.045, and the was 10.4444. The fixation index (F) value between and was 0.9207. Furthermore, the F value within (0.0275) was significantly lower than that within (0.2106), indicating a greater genetic variation of compared with . In conclusion, the genetic diversity analysis based on RT1-Db1exon1 showed that had higher gene polymorphism and greater genetic differentiation compared with in the Junggar Basin plague focus, which might be associated with the low infection rate of .
在准噶尔盆地鼠疫疫源地，[物种A]和[物种B]生活在一起，且它们的寄生蚤具有相似的群落结构。然而，[物种A]的[某种指标]和抗F1抗体阳性率显著高于[物种B]。在本研究中，在准噶尔盆地东部的奇台县、阜康市和木垒县采集了[物种A]和抗F1抗体阴性的[物种B]。从它们的脾脏组织中提取基因组DNA，通过PCR程序扩增RT1-Db1exon1，然后进行测序。进行序列分析并计算和比较分子多样性参数。结果表明，[物种A]和[物种B]在核苷酸组成、氨基酸组成、单核苷酸多态性（SNP）位点的数量和分布以及单倍型数量上存在显著差异。[物种A]的核苷酸多样性（π）为0.00420±0.00139，单倍型多样性（Hd）为0.833±0.086，核苷酸差异平均数（k）为2.02564。[物种B]的π为0.06569±0.02524，Hd为1.000±0.045，k为10.4444。[物种A]和[物种B]之间的固定指数（F）值为0.9207。此外，[物种A]内部的F值（0.0275）显著低于[物种B]内部的F值（0.2106），表明[物种A]与[物种B]相比具有更大的遗传变异。总之，基于RT1-Db1exon1的遗传多样性分析表明，在准噶尔盆地鼠疫疫源地，[物种A]与[物种B]相比具有更高的基因多态性和更大的遗传分化，这可能与[物种B]的低感染率有关。
