College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, 712100, China; Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture, Yangling, 712100, Shaanxi, PR China.
College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Chemosphere. 2022 Mar;291(Pt 2):132813. doi: 10.1016/j.chemosphere.2021.132813. Epub 2021 Nov 6.
Selenium (Se) deficiency has been a public health concern for years. Arbuscular mycorrhizal fungi (AMF) play an essential role in improving Se uptake in crops, but related mechanisms still remain unclear. To explore the influence of AMF on uptake of Se in winter wheat, a pot experiment was conducted to inoculate wheat with Funneliformis mosseae (F.m) or not under different levels of selenate in soil. The present results indicated that inoculation of F.m significantly (p < 0.05) increased Se concentration in shoots and roots of wheat under low level of selenate (≤5.0 mg kg) treatments, while the contrary pattern was recorded under high level of selenate (15 and 20 mg kg) treatments. Moreover, inoculation of F.m significantly increased concentration of available Se in soil by 4.68-34.05%. Under selenate ≤5 mg kg treatments, the expression of TaeSultr1;1 and TaeSultr1;3 in roots of mycorrhizal wheat was significantly up-regulated by 3.06-5.53 and 0.63-5.12 times, while reached saturation under selenate >5 mg kg treatments. In addition, partial least squares path modeling (PLS-PM) showed that inoculation of AMF directly affected the expression of sulfate transporter and that both sulfate transporter and soil Se fractions played a significant positive effect on plant Se content. The present study indicated that AMF on Se concentration in winter wheat depends on the level of selenate spiked in soil and added to our understanding of the functions and applications of AMF on crop Se absorption.
多年来，硒（Se）缺乏一直是公共卫生关注的问题。丛枝菌根真菌（AMF）在提高作物硒吸收方面起着至关重要的作用，但相关机制仍不清楚。为了探索 AMF 对冬小麦硒吸收的影响，进行了一项盆栽实验，在土壤中添加不同水平的硒酸盐条件下，接种或不接种摩西管柄囊霉（F.m）。本研究结果表明，在低水平硒酸盐（≤5.0mgkg）处理下，接种 F.m 显著（p<0.05）增加了冬小麦地上部和根系中的硒浓度，而在高水平硒酸盐（15 和 20mgkg）处理下则相反。此外，接种 F.m 可使土壤中有效硒的浓度增加 4.68-34.05%。在硒酸盐≤5mgkg 处理下，接种 AMF 可使根中 TaeSultr1;1 和 TaeSultr1;3 的表达分别显著上调 3.06-5.53 倍和 0.63-5.12 倍，而在硒酸盐>5mgkg 处理下达到饱和。此外，偏最小二乘路径模型（PLS-PM）表明，AMF 对硫酸盐转运蛋白的表达有直接影响，硫酸盐转运蛋白和土壤硒形态均对植物硒含量有显著的正影响。本研究表明，AMF 对冬小麦硒浓度的影响取决于土壤中添加的硒酸盐水平，增加了我们对 AMF 促进作物硒吸收功能和应用的理解。
