Zhang Zengyu, Li Bei, Liu Yongxian, He Lixin, Pang Ting, Chen Zongdao, Shohag Md Jahidul Islam, Miao Xiuyan, Li Xi, Gu Minghua, Wei Yanyan
Cultivation Base of Guangxi Key Laboratory for Agro-Environment and Agro-Products Safety, State Key Laboratory for Conservation and Utilization of Subtropical Agri-Bioresources, College of Agriculture, Guangxi University, Nanning 530004, China.
Guangxi Academy of Agricultural Sciences, Nanning 530004, China.
Toxics. 2022 Sep 26;10(10):565. doi: 10.3390/toxics10100565.
Selenium (Se) is an essential trace element for humans. Arbuscular mycorrhizal fungi (AMF) play a crucial role in increasing plant micronutrient acquisition. Soybean ( (Linn.) Merr.) is a staple food for most people around the world and a source of Se. Therefore, it is necessary to study the mechanism of Se intake in soybean under the influence of AMF. In this study, the effects of fertilization with selenite and inoculation with different AMF strains ( (Ce), (Fm)) on the accumulation and speciation of Se in common soybean plants were discussed. We carried out a pot experiment at the soil for 90 days to investigate the impact of fertilization with selenite and inoculation with Ce and Fm on the Se fractions in soil, soybean biomass, accumulation and speciation of Se in common soybean plants. The daily dietary intake of the Se (DDI) formula was used to estimate the risk threshold of human intake of Se from soybean seeds. The results showed that combined use of both AMF and Se fertilizer could boost total Se and organic Se amounts in soyabean seeds than that of single Se application and that it could increase the proportion of available Se in soil. Soybean inoculated with Fm and grown in soil fertilized with selenite had the highest organic Se. The results suggest that AMF inoculation could promote root growth, more soil water-soluble Se and higher Se uptake. The maximum Se intake of soybean for adults was 93.15 μg/d when treated with Se fertilizer and Fm, which satisfies the needs of Se intake recommended by the WHO. Combined use of AMF inoculation and Se fertilizer increases the bioavailable Se in soil and promotes the total Se concentration and organic Se accumulation in soybean. In conclusion, AMF inoculation combined with Se fertilization can be a promising strategy for Se biofortification in soybean.
硒（Se）是人体必需的微量元素。丛枝菌根真菌（AMF）在增加植物微量营养素获取方面发挥着关键作用。大豆（(Linn.) Merr.）是世界上大多数人的主食和硒的来源。因此，有必要研究AMF影响下大豆吸收硒的机制。本研究探讨了亚硒酸盐施肥和接种不同AMF菌株（(Ce)、(Fm)）对普通大豆植株中硒积累和形态的影响。我们在土壤中进行了为期90天的盆栽试验，以研究亚硒酸盐施肥以及接种Ce和Fm对土壤中硒组分、大豆生物量、普通大豆植株中硒的积累和形态的影响。采用硒的每日膳食摄入量（DDI）公式来估计人类从大豆种子中摄入硒的风险阈值。结果表明，与单独施用硒相比，AMF和硒肥联合使用可提高大豆种子中的总硒和有机硒含量，并能增加土壤中有效硒的比例。接种Fm并在亚硒酸盐施肥土壤中生长的大豆有机硒含量最高。结果表明，接种AMF可促进根系生长，增加土壤水溶性硒含量并提高硒吸收量。当用硒肥和Fm处理时，成年人食用大豆的最大硒摄入量为93.15μg/d，满足了世界卫生组织推荐的硒摄入量需求。AMF接种和硒肥联合使用增加了土壤中生物可利用硒的含量，促进了大豆中总硒浓度和有机硒的积累。总之，接种AMF并结合硒肥可能是大豆硒生物强化的一种有前景的策略。
