Qin Yan, Cai Qiuliang, Ling Yiting, Chen Xue, Xu Jingmao, Huang Guirong, Liang Shanhe, Yuan Xiu, Yang Xiao Mu, Lu Dan, Wang Xueli, Wei Yanyan
State Key Laboratory for Conservation and Utilization of Subtropical Agri-bioresources, Guangxi Key Laboratory for Agro-Environment and Agro-Products Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, China.
Industrial College of Subtropical Characteristic Agriculture, Agriculture and Food Engineering College, Baise University, Baise, China.
Front Plant Sci. 2023 Sep 20;14:1242463. doi: 10.3389/fpls.2023.1242463. eCollection 2023.
Although selenium (Se) is an essential trace element in humans, the intake of Se from food is still generally inadequate throughout the world. Inoculation with arbuscular mycorrhizal fungi (AMF) improves the uptake of Se in rice ( L.). However, the mechanism by which AMF improves the uptake of Se in rice at the transcriptome level is unknown. Only a few studies have evaluated the effects of uptake of other elements in rice under the combined effects of Se and AMF. In this study, Se combined with the AMF (Fm) increased the biomass and Se concentration of rice plants, altered the pattern of ionomics of the rice roots and shoots, and reduced the antagonistic uptake of Se with nickel, molybdenum, phosphorus, and copper compared with the treatment of Se alone, indicating that Fm can enhance the effect of fertilizers rich in Se. Furthermore, a weighted gene co-expression network analysis (WGCNA) showed that the hub genes in modules significantly associated with the genes that contained Se and were related to protein phosphorylation, protein serine/threonine kinase activity, membrane translocation, and metal ion binding, suggesting that the uptake of Se by the rice roots may be associated with these genes when Fm and Se act in concert. This study provides a reference for the further exploration of genes related to Se uptake in rice under Fm treatment.
虽然硒（Se）是人体必需的微量元素，但全球范围内通过食物摄入的硒普遍仍不充足。接种丛枝菌根真菌（AMF）可提高水稻（L.）对硒的吸收。然而，在转录组水平上，AMF提高水稻对硒吸收的机制尚不清楚。仅有少数研究评估了在硒和AMF共同作用下水稻对其他元素吸收的影响。在本研究中，与单独施用硒处理相比，硒与AMF（Fm）组合增加了水稻植株的生物量和硒浓度，改变了水稻根和地上部的离子组学模式，并减少了硒与镍、钼、磷和铜的拮抗吸收，表明Fm可增强富硒肥料的效果。此外，加权基因共表达网络分析（WGCNA）表明，模块中的枢纽基因与含硒基因显著相关，且与蛋白质磷酸化、蛋白质丝氨酸/苏氨酸激酶活性、膜转运和金属离子结合有关，这表明当Fm和硒共同作用时，水稻根对硒的吸收可能与这些基因有关。本研究为进一步探索Fm处理下水稻中与硒吸收相关的基因提供了参考。
