Linkage analysis of the murine interferon alpha locus (Ifa) on chromosome 4.

We performed linkage analysis of the murine interferon alpha gene cluster, Ifa, with three different marker genes on chromosome 4: the major urinary protein locus (Mup-1), the brown locus (b) and the misty locus (m). The gene order (from centromere) with intervening percent recombination derived from a first three-point cross is Mup-1--13.6 (+/- 3.6)--Ifa--7.9 (+/- 2.8)--m and from a second three-point cross Mup-1--1.7 (+/- 1.7)--b--3.5 (+/- 2.4)--Ifa. The combined results indicate that the gene order, from the centromere, is Mup-1--b--Ifa--m.