Dandoy F, Kelley K A, DeMaeyer-Guignard J, DeMaeyer E, Pitha P M
J Exp Med. 1984 Jul 1;160(1):294-302. doi: 10.1084/jem.160.1.294.
Southern blot analysis with a murine interferon-alpha2 (MuIFN-alpha2) cDNA probe revealed restriction fragment polymorphism of EcoRI- and HindIII-digested C57BL/6 and BALB/cDNA. The inheritance pattern of this polymorphism was examined using DNA from each of the seven recombinant inbred strains derived from C57BL/6 and BALB/c; the strain distribution pattern suggests linkage of INF-alpha genes to two histocompatibility loci on chromosome 4. Southern blot analysis of DNA from six bilinear congenic strains carrying different fragments of the BALB/c chromosome 4 on a C57BL/6 background showed linkage of IFN-alpha genes to the histocompatibility locus H-15. It can therefore be concluded that the IFN-alpha gene cluster is situated on chromosome 4 near the H-15 locus, between loci Mup-1 and b.
用鼠干扰素α2（MuIFN-α2）cDNA探针进行的Southern印迹分析显示，经EcoRI和HindIII酶切的C57BL/6和BALB/cDNA存在限制性片段多态性。利用来自C57BL/6和BALB/c的七个重组近交系各自的DNA检测了这种多态性的遗传模式；菌株分布模式表明INF-α基因与4号染色体上的两个组织相容性位点连锁。对在C57BL/6背景上携带BALB/c 4号染色体不同片段的六个双线性同源近交系的DNA进行Southern印迹分析，结果显示IFN-α基因与组织相容性位点H-15连锁。因此可以得出结论，IFN-α基因簇位于4号染色体上靠近H-15位点处，在Mup-1和b位点之间。
