Ali Rizwan, El-Boubbou Kheireddine, Boudjelal Mohamed
Medical Research Core Facility and Platforms (MRCFP), King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences (KSAU-HS), Ministry of National Guard Health Affairs (MNGHA), Riyadh 11481, Kingdom of Saudi Arabia.
Department of Basic Sciences, College of Science & Health Professions (COSHP), King Saud bin Abdulaziz University for Health Sciences (KSAU-HS), King Abdulaziz Medical City, National Guard Health Affairs, Riyadh 11481, Saudi Arabia.
MethodsX. 2021 Sep 20;8:101521. doi: 10.1016/j.mex.2021.101521. eCollection 2021.
Biological samples usually require cumbersome preparation steps before SEM imaging. Here we propose a simple, fast and inexpensive method to prepare and visualize biological cell culture samples in a few easy steps. We have tested this method with success on several adherent breast cancer and non-adherent leukemia cell lines. This method gives results comparable to other well-established techniques, and it can be convenient in day-to-day biological sample preparation for SEM imaging.•An easy and rapid method to visualize biological specimens under SEM.•Cells are grown on carbon tapes and gold coated.•Air drying without compromising the image quality.
在进行扫描电子显微镜（SEM）成像之前，生物样本通常需要繁琐的制备步骤。在此，我们提出一种简单、快速且经济的方法，通过几个简单步骤来制备和观察生物细胞培养样本。我们已在几种贴壁乳腺癌细胞系和非贴壁白血病细胞系上成功测试了该方法。此方法所得结果与其他成熟技术相当，且在日常用于SEM成像的生物样本制备中非常便捷。
•一种在SEM下观察生物标本的简便快速方法。
•细胞在碳带上生长并进行金涂层处理。
•空气干燥且不影响图像质量。
